US 6,982,083 B1 | ||
Starch granules containing a recombinant polypeptide of interest, method for obtaining them, and their uses | ||
Christophe D'Hulst, Wattrelos (France); and Steven Ball, Bourghelles (France) | ||
Assigned to Centre National de la Recherche Scientifique, (France) | ||
Appl. No. 9/980,771 PCT Filed May 19, 2000, PCT No. PCT/FR00/01384 § 371(c)(1), (2), (4) Date Jan. 10, 2002, PCT Pub. No. WO00/71734, PCT Pub. Date Nov. 30, 2000. |
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Claims priority of application No. 99 06494 (FR), filed on May 21, 1999. | ||
Int. Cl. A61K 38/51 (2006.01); C12N 9/00 (2006.01); C12N 9/10 (2006.01); C12N 1/20 (2006.01); C07H 21/04 (2006.01) |
U.S. Cl. 424—94.5 | 13 Claims |
1. A pharmaceutical composition, comprising starch granules containing at least one fusion polypeptide comprising:
In the N terminal position:
the peptide sequence of SEQ ID No: 3 comprising the granule bound starch synthase GBSSI of Chlamydomonas reinhardtii or the sequence SEQ ID No: 5 comprising the GBSSI of Chlamydomonas reinhardtii in the form of mature protein of 651 amino acids, said sequences being encoded by nucleotide sequences SEQ ID No: 2, and 4
respectively,
and, in the C-terminal position, a peptide or polypeptide of interest, the C-terminal part of the amino acid sequence of the
GBSSI thus being bound to the N-terminal part of the peptide sequence of interest,
the said fusion polypeptide being encoded by a recombinant nucleotide sequence containing in the 5′→3′ direction, a nucleotide
sequence coding for said Chlamydomonas reinhardtii GBSSI,
the said nucleotide sequence coding for this enzyme being positioned upstream of a nucleotide sequence coding for the peptide
or polypeptide of interest, the peptide of interest in the said fusion polypeptides possessing a defined therapeutic effect.
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