US 6,982,158 B2
Extraction and purification of phosphodiesterase 1
Michael Patane, Seaforth (Australia)
Assigned to Protech Research Pty Ltd, Seaforth (Australia)
Filed on Dec. 11, 2003, as Appl. No. 10/733,857.
Claims priority of application No. 2003902303 (AU), filed on May 14, 2003.
Prior Publication US 2004/0229332 A1, Nov. 18, 2004
Int. Cl. C12N 9/22 (2006.01)
U.S. Cl. 435—199 12 Claims
 
1. A process for purifying a phosphodiesterase 1 (PDE-1) from a cell including heating an extract of a cell formed from a solution including at least one divalent cation, to increase the specific activity of PDE-1 in the extract, wherein the concentration of the divalent cation is about 50 mM.