US 6,982,165 B2 | ||
Nucleic acid sequencing by raman monitoring of molecular deconstruction | ||
Mineo Yamakawa, Campbell, Calif. (US); Andrew Berlin, San Jose, Calif. (US); Steve Kirch, Pleasanton, Calif. (US); Gabi Neubauer, Los Gatos, Calif. (US); and Valluri Rao, Saratoga, Calif. (US) | ||
Assigned to Intel Corporation, Santa Clara, Calif. (US) | ||
Filed on Sep. 24, 2001, as Appl. No. 9/962,555. | ||
Prior Publication US 2003/0058799 A1, Mar. 27, 2003 | ||
Int. Cl. C12M 1/34 (2006.01); C12Q 1/68 (2006.01) |
U.S. Cl. 435—287.2 | 16 Claims |
1. An apparatus comprising:
a) a reaction chamber to contain an immobilization surface, the immobilization surface to attach one or more unlabeled nucleic
acid molecules;
b) a flow path attached to the reaction chamber;
c) a liquid in the flow path;
d) one or more unlabeled nucleotides free in solution in the liquid at the point of nucleotide detection; and
e) a detection unit programmed to detect only the unlabeled nucleotides free in a liquid solution as they move past the detection
unit, the detection unit comprising an excitation source and a Raman spectroscopic detector.
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