US 6,982,147 B2 | ||
Apparatus for assaying biopolymer binding by means of multiple measurements under varied conditions | ||
Glen H. Erikson, Providenciales (Turks and Caicos Islands) | ||
Assigned to Ingeneus Corporation, Bridgetown (Barbados) | ||
Filed on Apr. 10, 2002, as Appl. No. 10/120,092. | ||
Application 10/120092 is a continuation in part of application No. 09/911042, filed on Jul. 23, 2001. | ||
Application 09/911042 is a continuation in part of application No. 09/490273, filed on Jan. 24, 2000, granted, now 6,265,170, filed on Jul. 24, 2001. | ||
Prior Publication US 2002/0123066 A1, Sep. 05, 2002 | ||
Int. Cl. C12Q 1/68 (2006.01); C12N 15/00 (2006.01); C12N 15/63 (2006.01); C12N 1/20 (2006.01); C07H 21/04 (2006.01) |
U.S. Cl. 435—6 | 19 Claims |
1. An apparatus for assaying specific binding of a probe to a target, said apparatus comprising:
a sample support for supporting a sample containing the probe and the target;
a light source for irradiating the sample;
an optical train for conveying light from the light source to the sample;
a light detector for detecting light emitted from the sample;
an electricity source for providing an electric charge through the sample;
an electrical property detector for detecting an electrical property of the sample; and
a data analysis device in communication with the light detector and the electrical property detector, wherein the data analysis
device is adapted to:
(a) (1) produce an optical determination of probe-target binding as a function of light emitted from the sample,
(2) produce an electrical determination of probe-target binding as a function of the electrical property, and
(3) compare the optical determination with the electrical determination to assay specific binding of the probe to the target;
or
(b) (1) produce a pre-electrification determination of probe-target binding as a function of light emitted from the sample
prior to providing the electric charge through the sample,
(2) produce a post-electrification determination of probe-target binding as a function of light emitted from the sample concurrent
with and/or subsequent to providing the electric charge through the sample, and
(3) compare the pre-electrification determination with the post-electrification determination to assay specific binding of
the probe to the target.
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