US 6,982,150 B2 | ||
System and method for identifying proteins involved in force-initiated signal transduction | ||
Michael P. Sheetz, 560 Riverside Dr., Apt. 7b, New York, N.Y. 10027 (US); and Yasuhiro Sawada, 54 Morningside Dr., Greenwich, Conn. 06830 (US) | ||
Filed on Aug. 10, 2004, as Appl. No. 10/914,924. | ||
Application 10/914924 is a continuation of application No. PCT/US02/15794, filed on May 16, 2002. | ||
Claims priority of provisional application 60/355927, filed on Feb. 11, 2002. | ||
Prior Publication US 2005/0084843 A1, Apr. 21, 2005 | ||
Int. Cl. G01N 33/53 (2006.01) |
U.S. Cl. 435—7.2 | 53 Claims |
26. A method of identifying proteins involved in mechanotransduction comprising:
providing a cytoskeleton network deficient in force-dependent proteins;
contacting the cytoskeleton network with exogenous proteins,
applying a force to the cytoskeleton network, and
identifying the exogenous proteins that bind to the cytoskeleton network when the force is applied thereto, wherein the exogenous
proteins that bind to the cytoskeleton network are identified as proteins involved in mechanotransduction.
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