US 6,982,146 B1
High speed parallel molecular nucleic acid sequencing
Thomas D. Schneider, Frederick, Md. (US); and Denise Rubens, Yardville, N.J. (US)
Assigned to The United States of America as represented by the Department of Health and Human Services, Washington, D.C. (US)
Appl. No. 10/70,053
PCT Filed Aug. 29, 2000, PCT No. PCT/US00/23736
§ 371(c)(1), (2), (4) Date Jun. 10, 2002,
PCT Pub. No. WO01/16375, PCT Pub. Date Mar. 08, 2001.
Claims priority of provisional application 60/151580, filed on Aug. 30, 1999.
Int. Cl. C12Q 1/68 (2006.01); C12P 19/34 (2006.01); C07H 21/02 (2006.01); C07H 21/04 (2006.01)
U.S. Cl. 435—6 38 Claims
OG exemplary drawing
 
1. A method of sequencing a sample nucleic acid molecule, comprising:
exposing the sample nucleic acid molecule to an oligonucleotide primer and a polymerase in the presence of a mixture of different nucleotides, wherein the polymerase and the nucleotides each comprise a fluorophore which emits a signal corresponding to addition of a particular nucleotide as each nucleotide is incorporated into a synthesized nucleic acid molecule which is complementary to the sample nucleic acid molecule; and
detecting the signal as each nucleotide is incorporated into the synthesized nucleic acid molecule.