US 6,982,163 B2
Process for preparing beta-fructofuranosidase enzyme and a process for producing fructooligosaccharides
Yong Kun Park, Campinas (Brazil); and Glaucia Maria Pastores, Campinas (Brazil)
Assigned to Usina Da Barra S/A-Acucar e Alcool, Barra Bonita (Brazil)
Filed on Sep. 30, 2002, as Appl. No. 10/260,799.
Application 10/260799 is a continuation of application No. 09/535510, filed on Mar. 24, 2000, abandoned.
Application 09/535510 is a continuation of application No. 09/194456, abandoned, previously published as PCT/BR98/00022, filed on Mar. 24, 1998.
Claims priority of application No. 9700452 (BR), filed on Mar. 25, 1997.
Prior Publication US 2003/0082750 A1, May 01, 2003
Int. Cl. C12N 1/14 (2006.01); C12N 9/30 (2006.01); C12N 9/62 (2006.01); C12N 1/00 (2006.01); C12P 1/02 (2006.01)
U.S. Cl. 435—254.3 2 Claims
 
1. A process for preparing a beta-fructofuranosidase enzyme comprising the steps of:
a) inoculating the spores of the fungus Aspergillus niger 489 having the deposit designation of PTA-5032 in a liquid or semi-solid culture medium;
b) cultivating the inoculated fungus of step (a), in order to promote its growth with the formation of mycelium and the production of the beta-fructofuranosidase enzyme; and
c) separating the beta-fructofuranosidase enzyme from the mycelium and from the culture medium.