US 6,982,144 B2
Thermostable polymerases having altered fidelity and methods of identifying and using same
Lawrence A. Loeb, Bellevue, Wash. (US); Leroy Hood, Seattle, Wash. (US); and Motoshi Suzuki, Nogoya (Japan)
Assigned to University of Washington, Seattle, Wash. (US)
Filed on Oct. 04, 2001, as Appl. No. 9/972,834.
Application 09/972834 is a continuation of application No. 08/978806, filed on Nov. 26, 1997.
Claims priority of provisional application 60/031496, filed on Nov. 27, 1996.
Prior Publication US 2002/0192663 A1, Dec. 19, 2002
This patent is subject to a terminal disclaimer.
Int. Cl. C12Q 1/68 (2006.01); C12N 9/10 (2006.01); C07K 1/00 (2006.01)
U.S. Cl. 435—6 16 Claims
 
1. A composition comprising a Taq DNA polymerase mutant, wherein said polymerase mutant comprises one or more amino acid substitutions selected from the group consisting of Phe667Leu; Asn666Asp; Asn666Ile; Ile665Leu; Leu670Val; Arg660Tyr; Arg660Ser; Gly668Arg; Arg660Lys; Gly668Ser; Gly668Gln; Thr664Ile and Asn666Asp; Ala661Ser and Val669Leu; Ala661Glu, Ile665Thr, and Phe667Leu; and Thr664Pro, Ile665Val and Asn666Tyr, and a template neucleic acid molecule.