US 6,982,143 B1
Normalizing and amplifying RNA
John Ngai, Berkeley, Calif. (US); and David Lin, Berkeley, Calif. (US)
Assigned to The Regents of the University of California, Oakland, Calif. (US)
Filed on Jun. 20, 2000, as Appl. No. 9/597,608.
Int. Cl. C12Q 1/68 (2006.01); C12Q 1/48 (2006.01); C12P 19/34 (2006.01); C07H 21/04 (2006.01)
U.S. Cl. 435—6 19 Claims
 
1. A method for normalizing and amplifying an RNA population comprising the steps of:
copying message RNA (mRNA) to form first single-stranded (ss) cDNA;
converting the first ss-cDNA to first double-stranded (ds) cDNA;
linearly amplifying the first ds-cDNA to form first amplified RNA (aRNA);
tagging the 3′ end of the first aRNA with a known sequence to form 3′-tagged first aRNA;
copying the 3′-tagged first aRNA to form second ss-cDNA; and
normalizing the mRNA or the first aRNA.