US 6,982,169 B2
Chemical inhibitors of mismatch repair
Nicholas C. Nicolaides, Boothwyn, Pa. (US); Luigi Grasso, Philadelphia, Pa. (US); and Philip M. Sass, Audubon, Pa. (US)
Assigned to Morphotek, Inc., Exton, Pa. (US)
Filed on Jan. 15, 2001, as Appl. No. 9/760,285.
Prior Publication US 2003/0091997 A1, May 15, 2003
Int. Cl. C12N 15/00 (2006.01); C12N 1/16 (2006.01); A01N 27/00 (2006.01)
U.S. Cl. 435—325 29 Claims
 
1. A method for making a hypermutable plant or yeast cell in vitro comprising exposing a plant cell or yeast cell to an inhibitor of mismatch repair, thereby rendering said cell hypermutable, wherein said inhibitor is an anthracene, wherein said anthracene has the formula:
OG Complex Work Unit Drawing
wherein R1-R10 are independently hydrogen, hydroxyl, amino group, alkyl, substituted alkyl, alkenyl, substituted alkenyl, alkynyl, substituted alkynyl, O-alkyl, S-alkyl, N-alkyl, O-alkenyl, S-alkenyl, N-alkenyl, O-alkynyl, S-alkynyl, N-alkynyl, aryl, substituted aryl, aryloxy, substituted aryloxy, heteroaryl, substituted heteroaryl, aralkyloxy, arylalkyl, alkylaryl, alkylaryloxy, arylsulfonyl, alkylsulfonyl, alkoxycarbonyl, aryloxycarbonyl, guanidino, carboxy, an alcohol, an amino acid, sulfonate, alkyl sulfonate, CN, NO2, an aldehyde group, an ester, an ether, a crown ether, a ketone, an organosulfur compound, an organometallic group, a carboxylic acid, an organosilicon or a carbohydrate that optionally contains one or more alkylated hydroxyl groups;
wherein said heteroaryl, and substituted heteroaryl contain at least one heteroatom that is oxygen, sulfur, a metal atom, phosphorus, silicon or nitrogen; and
wherein said substituents of the substituted alkyl, substituted alkenyl, substituted alkynyl, substituted aryl, and substituted heteroaryl are halogen, CN, NO2, lower alkyl, aryl, heteroaryl, aralkyl, aralkyloxy, guanidino, alkoxycarbonyl, alkoxy, hydroxy, carboxy or amino group; and
wherein said amino group is optionally substituted with an acyl group, or 1 to 3 aryl or lower alkyl groups.