US 6,982,083 B1
Starch granules containing a recombinant polypeptide of interest, method for obtaining them, and their uses
Christophe D'Hulst, Wattrelos (France); and Steven Ball, Bourghelles (France)
Assigned to Centre National de la Recherche Scientifique, (France)
Appl. No. 9/980,771
PCT Filed May 19, 2000, PCT No. PCT/FR00/01384
§ 371(c)(1), (2), (4) Date Jan. 10, 2002,
PCT Pub. No. WO00/71734, PCT Pub. Date Nov. 30, 2000.
Claims priority of application No. 99 06494 (FR), filed on May 21, 1999.
Int. Cl. A61K 38/51 (2006.01); C12N 9/00 (2006.01); C12N 9/10 (2006.01); C12N 1/20 (2006.01); C07H 21/04 (2006.01)
U.S. Cl. 424—94.5 13 Claims
 
1. A pharmaceutical composition, comprising starch granules containing at least one fusion polypeptide comprising:
In the N terminal position:
the peptide sequence of SEQ ID No: 3 comprising the granule bound starch synthase GBSSI of Chlamydomonas reinhardtii or the sequence SEQ ID No: 5 comprising the GBSSI of Chlamydomonas reinhardtii in the form of mature protein of 651 amino acids, said sequences being encoded by nucleotide sequences SEQ ID No: 2, and 4 respectively,
and, in the C-terminal position, a peptide or polypeptide of interest, the C-terminal part of the amino acid sequence of the GBSSI thus being bound to the N-terminal part of the peptide sequence of interest,
the said fusion polypeptide being encoded by a recombinant nucleotide sequence containing in the 5′→3′ direction, a nucleotide sequence coding for said Chlamydomonas reinhardtii GBSSI,
the said nucleotide sequence coding for this enzyme being positioned upstream of a nucleotide sequence coding for the peptide or polypeptide of interest, the peptide of interest in the said fusion polypeptides possessing a defined therapeutic effect.