US 6,982,318 B1
Production of motif-specific and context-independent antibodies using peptide libraries as antigens
Michael J. Comb, Manchester, Mass. (US); and Yi Tan, Lynnfield, Mass. (US)
Assigned to Cell Signaling Technology, Inc., Beverly, Mass. (US)
Filed on Mar. 24, 2000, as Appl. No. 9/535,364.
Application 09/535364 is a continuation in part of application No. 09/148712, filed on Sep. 04, 1998, granted, now 6,441,140.
Claims priority of application No. PCT/US99/19597 (WO), filed on Aug. 26, 1999.
Int. Cl. C07K 16/18 (2006.01)
U.S. Cl. 530—387.1 11 Claims
 
1. A motif-specific, context-independent antibody that specifically binds a recurring kinase consensus substrate motif or a protein-protein binding motif, said motif consisting of up to four invariant amino acids, at least one phosphorylated amino acid, and optionally, one or more variable/degenerate amino acid position(s), said antibody specifically binding said motif in a plurality of different peptides or proteins from an organism in which said motif recurs, wherein said antibody is not a site-specific antibody.