US 7,320,877 B1
Dihydroorotase extracted from plants
Thomas Ehrhardt, Speyer (Germany); Jens Lerchl, Ladenburg (Germany); Marc Stitt Nigel, Edingen-Neckarhausen (Germany); Rita Zrenner, Ladenburg (Germany); and Michael Schroeder, Mannheim (Germany)
Assigned to BASF Aktiengesellschaft, Ludwigshafen (Germany)
Appl. No. 10/70,277
PCT Filed Sep. 02, 2000, PCT No. PCT/EP00/08581
§ 371(c)(1), (2), (4) Date Mar. 06, 2002,
PCT Pub. No. WO01/18190, PCT Pub. Date Mar. 15, 2001.
Claims priority of application No. 199 42 742 (DE), filed on Sep. 07, 1999.
Int. Cl. C12Q 1/34 (2006.01); C12N 9/14 (2006.01); C12N 1/20 (2006.01); C12N 15/00 (2006.01)
U.S. Cl. 435—18  [435/195; 435/252.3; 435/320.1; 536/23.2; 530/350] 17 Claims
 
1. A method for screening herbicidally active substances which inhibit the activity of plant dihydroorotase, comprising:
generating, in a first step, dihydroorotase or a protein having the enzymatic activity of a dihydroorotase, and
in a second step, measuring activity of the dihydroorotase in the presence and absence of a test substance, wherein the dihydroorotase or protein is generated from the expression of a DNA sequence having a homology of at least 80% with SEQ ID NO:1.