US 7,320,870 B2 | ||
Ocular tear growth factor-like protein | ||
Gordon W. Laurie, Charlottesville, Va. (US); Sandhya Sanghi, Temple, Tex. (US); Kumar Rajesh, Temple, Tex. (US); and Angela J. Lumsden, Mosman Park (Australia) | ||
Assigned to University of Virginia Patent Foundation, Charlottesville, Va. (US) | ||
Filed on Mar. 06, 2007, as Appl. No. 11/714,586. | ||
Application 11/714586 is a division of application No. 10/468372, previously published as PCT/US02/04971, filed on Feb. 20, 2002. | ||
Claims priority of provisional application 60/269900, filed on Feb. 20, 2001. | ||
Prior Publication US 2007/0167372 A1, Jul. 19, 2007 | ||
Int. Cl. G01N 33/53 (2006.01) |
U.S. Cl. 435—7.1 [435/7.2; 435/69.1; 435/70.1; 530/350; 436/501] | 1 Claim |
1. A method for identifying a nucleic acid sequence encoding a lacritin receptor, said method comprising providing cells that
have been transfected with nucleic acid sequences that encode for potential cell receptors;
contacting said transfected cells with lacritin (SEQ ID NO:4);
detecting cells that display lacritin-dependent calcium signaling;
isolating nucleic acid sequences from the detected cells; and
identifying a nucleic acid sequence that confers lacritin-dependent calcium signaling on cells transfected with that nucleic
acid sequence.
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