US 7,320,882 B2 | ||
Process for L-amino acid production using enterobacteriaceae strain with enhanced ptsG expression | ||
Mechthild Rieping, Bielefeld (Germany) | ||
Assigned to Degussa AG, Dusseldorf (Germany) | ||
Appl. No. 10/481,743 PCT Filed Jun. 14, 2002, PCT No. PCT/EP02/06563 § 371(c)(1), (2), (4) Date May 07, 2004, PCT Pub. No. WO03/004670, PCT Pub. Date Jan. 16, 2003. |
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Claims priority of application No. 101 32 946 (DE), filed on Jul. 06, 2001. | ||
Prior Publication US 2004/0241812 A1, Dec. 02, 2004 | ||
Int. Cl. C12P 13/04 (2006.01); C12N 1/20 (2006.01) |
U.S. Cl. 435—106 [435/252.33] | 5 Claims |
1. A process for the preparation of L-amino acids in Enterobacteriaceae, comprising:
(a) fermenting, in a medium, Enterobacteriaceae which produce L-amino acid and in which the ptsG gene from Escherichia coli coding for glucose-specific IIBC component of the phosphotransferase system is over-expressed by increasing the copy number
of the ptsG gene,
(b) concentrating the L-amino acids in the medium or in the Enterobacteriaceae, and
(c) quantifying the L-amino acids.
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