US 7,320,874 B2
Assaying the teichoic acids of gram+ bacteria
Philippe Talaga, Sainte Consorce (France); Patricia Sepulcri, Marcy l'Etoile (France); and Sandrine Vialle-Blanc, Tassin la Demi Lune (France)
Assigned to Aventis Pasteur SA, Lyons (France)
Filed on Jul. 06, 2004, as Appl. No. 10/885,219.
Claims priority of provisional application 60/497653, filed on Aug. 25, 2003.
Claims priority of application No. 03 08305 (FR), filed on Jul. 08, 2003.
Prior Publication US 2005/0009121 A1, Jan. 13, 2005
Int. Cl. G01N 33/569 (2006.01); C07K 1/12 (2006.01)
U.S. Cl. 435—7.32  [530/343] 25 Claims
 
1. A method for quantifying the teichoic acids in a preparation comprising teichoic acids and one or more additional capsular polysaccharides of Streptococcus pneumoniae, the method comprising:
(i) treating the preparation with hydrofluoric acid (HF) at a temperature of less than or equal to 40° C., in order to release the oligosaccharides specific for the teichoic acids present in the preparation; and
(ii) quantifying the specific oligosaccharides obtained in (i).