US 7,321,843 B2
Method for generating new flow cytometry data files containing an infinite number of dimensions based on data estimation
José Alberto Orfao De Matos Correia E Vale, Salamanca (Spain); Carlos Eduardo Pedreira, Rio de Janeiro (Brazil); and Elaine Sobral Da Costa, Rio de Janerio (Brazil)
Assigned to Universidad de Salamanca, Salamanca (Spain)
Filed on Sep. 30, 2005, as Appl. No. 11/240,167.
Prior Publication US 2007/0078626 A1, Apr. 05, 2007
Int. Cl. G06F 15/00 (2006.01)
U.S. Cl. 702—182 40 Claims
OG exemplary drawing
 
1. A process for generating new flow cytometry data files with a potentially infinite number of dimensions, the data files containing events having associated information for each of a whole set of parameters evaluated, said information comprising measured data directly measured in a flow cytometer and data estimated after obtaining the measured data, comprising the steps of:
a) fusing two or more separate original flow cytometry data files containing information about events measured in different aliquots of a same sample, the original data files containing in common data about one or more parameters and information about one or more different parameters;
b) estimating a magnitude of the parameters and of a corresponding uncertainty measure for those parameters differing between one or more of the original data files, for each individual event contained in the fused data file, for which those variables were not directly measured in the flow cytometer and;
c) reconstructing multidimensionally a new data file containing both the measured data measured in the flow cytometer for each event of the individual original data files and the estimated data for those parameters not measured in a group of events from one or more of the original data files.