US 7,320,754 B2 | ||
Method of preparing multi-modal anion-exchange ligands | ||
Ulrika Carlsson, Uppsala (Sweden); Jean-Luc Maloisel, Uppsala (Sweden); and Nicolas Thevenin, Uppsala (Sweden) | ||
Assigned to GE Healthcare Bio-Sciences AB, Uppsala (Sweden) | ||
Appl. No. 10/547,567 PCT Filed Mar. 05, 2004, PCT No. PCT/SE2004/000317 § 371(c)(1), (2), (4) Date Aug. 29, 2005, PCT Pub. No. WO2004/078349, PCT Pub. Date Sep. 16, 2004. |
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Claims priority of application No. 0300625-1 (SE), filed on Mar. 05, 2003; and application No. 0300711-9 (SE), filed on Mar. 12, 2003. | ||
Prior Publication US 2006/0175237 A1, Aug. 10, 2006 | ||
This patent is subject to a terminal disclaimer. | ||
Int. Cl. B01D 15/08 (2006.01) |
U.S. Cl. 210—635 [210/656; 210/198.2; 210/502.1; 502/401; 502/439; 521/32; 521/33] | 9 Claims |
1. A method of preparing at least one multi-modal anion-exchange ligand, which method comprises the steps of
(a) providing at least one scaffold defined by the general formula (I) below
![]() wherein A, B and X irrespective of each other are carbon atoms or any heteroatoms, m is any integer between 0 and 4, and the
functional group N is a nitrogen, which either replaces one X or is attached to any one of A, B and X;
(b) optionally derivatizing the nitrogen of the scaffold(s) to provide an anion-exchanging group and/or to protect the amine,
while retaining the cyclic structure of the scaffold; and
(c) subjecting said scaffold to aminolysis to open up the cyclic structure of the resulting derivative by adding a reagent
comprising an amine coupled to a residue R to add said amine to the carbonyl carbon of the opened scaffold.
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