US 7,320,882 B2
Process for L-amino acid production using enterobacteriaceae strain with enhanced ptsG expression
Mechthild Rieping, Bielefeld (Germany)
Assigned to Degussa AG, Dusseldorf (Germany)
Appl. No. 10/481,743
PCT Filed Jun. 14, 2002, PCT No. PCT/EP02/06563
§ 371(c)(1), (2), (4) Date May 07, 2004,
PCT Pub. No. WO03/004670, PCT Pub. Date Jan. 16, 2003.
Claims priority of application No. 101 32 946 (DE), filed on Jul. 06, 2001.
Prior Publication US 2004/0241812 A1, Dec. 02, 2004
Int. Cl. C12P 13/04 (2006.01); C12N 1/20 (2006.01)
U.S. Cl. 435—106  [435/252.33] 5 Claims
OG exemplary drawing
 
1. A process for the preparation of L-amino acids in Enterobacteriaceae, comprising:
(a) fermenting, in a medium, Enterobacteriaceae which produce L-amino acid and in which the ptsG gene from Escherichia coli coding for glucose-specific IIBC component of the phosphotransferase system is over-expressed by increasing the copy number of the ptsG gene,
(b) concentrating the L-amino acids in the medium or in the Enterobacteriaceae, and
(c) quantifying the L-amino acids.