US 7,320,864 B2
Methods of using molecular constructs for detection of biochemical reactions
Jiacheng Yang, Hillsboro, N.J. (US)
Assigned to BioArray Solutions Ltd., Warren, N.J. (US)
Filed on Apr. 26, 2006, as Appl. No. 11/411,584.
Prior Publication US 2006/0234284 A1, Oct. 19, 2006
Int. Cl. C12Q 1/68 (2006.01); C12Q 1/00 (2006.01); C07H 21/02 (2006.01); C07H 21/04 (2006.01)
U.S. Cl. 435—6  [435/4; 536/23.1; 536/24.3] 7 Claims
 
1. A method of detecting a plurality of enzyme activities in a sample, comprising:
(a) adding a plurality of types of PNA-substrate chimerae to the sample, said PNA-substrate chimerae having a PNA capture portion and a substrate for an enzyme, wherein different types have different substrates capable of reacting with different enzymes but each type has a particular substrate and a selected PNA sequence capture portion;
(b) allowing the enzymes to react with the substrate portions of the chimera population;
(c) capturing the reacted PNA-substrate chimerae with different types of oligonucleotides, wherein said different types of oligonucleotides are displayed on differently encoded microparticles, and wherein said capturing is by annealing of a type of oligonucleotide to a selected PNA sequence capture portion; and
(d) detecting the changes in the substrates of captured reacted PNA-substrate chimerae;
(e) decoding the encoded microparticles to identify which substrates reacted and thereby identify the presence of particular enzymes in the sample.