US 7,320,884 B2
Fermentation process
Kevin W. Anderson, Indian Springs, Ohio (US); and J. Douglas Wenzel, Cincinnati, Ohio (US)
Assigned to Cognis Corporation, Ambler, Pa. (US)
Filed on Jan. 22, 2004, as Appl. No. 10/762,895.
Application 10/762895 is a continuation of application No. 09/663963, filed on Sep. 19, 2000, abandoned.
Claims priority of provisional application 60/156791, filed on Sep. 30, 1999.
Prior Publication US 2004/0152177 A1, Aug. 05, 2004
Int. Cl. C12P 7/44 (2006.01); C12N 1/18 (2006.01)
U.S. Cl. 435—142  [435/254.22] 11 Claims
 
1. A process for making a color and odor stable polycarboxylic acid or a polyhydoxy acid comprising:
(a) providing an organism for producing a polycarboxylic acid or a polyhydroxy acid;
(b) providing a substrate for conversion to a polycarboxylic acid or a polyhydroxy acid by the organism;
(c) providing a fermentation medium consisting of:
(i) a source of metabolizable carbon and energy;
(ii) a source of inorganic nitrogen selected from the group consisting of ammonia, ammonium hydroxide, ammonium sulphate, and mixtures thereof;
(iii) potassium phosphate;
(iv) at least one metal selected from the group consisting of an alkali metal, an alkaline earth metal, a transition metal, and mixtures thereof;
(v) a source of biotin, substantially free of particulate matter and bacteria;
(vi) at least one chelating agent; and
(vii) an antifoam agent; and
(d) fermenting a mixture comprising said organism, substrate and fermentation medium of (a), (b) and
(c) at a pH 5.4 to 7.