US 7,320,754 B2
Method of preparing multi-modal anion-exchange ligands
Ulrika Carlsson, Uppsala (Sweden); Jean-Luc Maloisel, Uppsala (Sweden); and Nicolas Thevenin, Uppsala (Sweden)
Assigned to GE Healthcare Bio-Sciences AB, Uppsala (Sweden)
Appl. No. 10/547,567
PCT Filed Mar. 05, 2004, PCT No. PCT/SE2004/000317
§ 371(c)(1), (2), (4) Date Aug. 29, 2005,
PCT Pub. No. WO2004/078349, PCT Pub. Date Sep. 16, 2004.
Claims priority of application No. 0300625-1 (SE), filed on Mar. 05, 2003; and application No. 0300711-9 (SE), filed on Mar. 12, 2003.
Prior Publication US 2006/0175237 A1, Aug. 10, 2006
This patent is subject to a terminal disclaimer.
Int. Cl. B01D 15/08 (2006.01)
U.S. Cl. 210—635  [210/656; 210/198.2; 210/502.1; 502/401; 502/439; 521/32; 521/33] 9 Claims
OG exemplary drawing
 
1. A method of preparing at least one multi-modal anion-exchange ligand, which method comprises the steps of
(a) providing at least one scaffold defined by the general formula (I) below

OG Complex Work Unit Drawing
wherein A, B and X irrespective of each other are carbon atoms or any heteroatoms, m is any integer between 0 and 4, and the functional group N is a nitrogen, which either replaces one X or is attached to any one of A, B and X;
(b) optionally derivatizing the nitrogen of the scaffold(s) to provide an anion-exchanging group and/or to protect the amine, while retaining the cyclic structure of the scaffold; and
(c) subjecting said scaffold to aminolysis to open up the cyclic structure of the resulting derivative by adding a reagent comprising an amine coupled to a residue R to add said amine to the carbonyl carbon of the opened scaffold.