US 7,320,864 B2 | ||
Methods of using molecular constructs for detection of biochemical reactions | ||
Jiacheng Yang, Hillsboro, N.J. (US) | ||
Assigned to BioArray Solutions Ltd., Warren, N.J. (US) | ||
Filed on Apr. 26, 2006, as Appl. No. 11/411,584. | ||
Prior Publication US 2006/0234284 A1, Oct. 19, 2006 | ||
Int. Cl. C12Q 1/68 (2006.01); C12Q 1/00 (2006.01); C07H 21/02 (2006.01); C07H 21/04 (2006.01) |
U.S. Cl. 435—6 [435/4; 536/23.1; 536/24.3] | 7 Claims |
1. A method of detecting a plurality of enzyme activities in a sample, comprising:
(a) adding a plurality of types of PNA-substrate chimerae to the sample, said PNA-substrate chimerae having a PNA capture
portion and a substrate for an enzyme, wherein different types have different substrates capable of reacting with different
enzymes but each type has a particular substrate and a selected PNA sequence capture portion;
(b) allowing the enzymes to react with the substrate portions of the chimera population;
(c) capturing the reacted PNA-substrate chimerae with different types of oligonucleotides, wherein said different types of
oligonucleotides are displayed on differently encoded microparticles, and wherein said capturing is by annealing of a type
of oligonucleotide to a selected PNA sequence capture portion; and
(d) detecting the changes in the substrates of captured reacted PNA-substrate chimerae;
(e) decoding the encoded microparticles to identify which substrates reacted and thereby identify the presence of particular
enzymes in the sample.
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