US 7,320,888 B2
Submergence-induced protein-like factors
Chau-Ting Yeh, Liver Research Unit, Chang Gung Memorial Hospital, 199 Tung Hwa North Road, Taipei 105 (Taiwan)
Filed on May 02, 2005, as Appl. No. 11/119,995.
Application 11/119995 is a division of application No. 10/236723, filed on Sep. 05, 2002.
Prior Publication US 2006/0019271 A1, Jan. 26, 2006
Int. Cl. C12N 7/00 (2006.01); C07K 5/00 (2006.01)
U.S. Cl. 435—235.1  [530/350] 1 Claim
 
1. A method of producing hepatitis C viruses, the method comprising infecting a human embryonic kidney cell line 293 expressing an Espstein-Barr nuclear antigen-1 gene and containing and expressing a nucleic acid encoding a polypeptide having SEQ ID NO:5, with a hepatitis C virus and allowing the virus to replicate in the said kidney cell line, wherein the polypeptide, when expressed in the said kidney cell line, increases susceptibility of the said kidney cell line to hepatitis C virus infection.