US 7,320,857 B2
Characterization of the earliest stages of the severe acute respiratory syndrome (SARS) virus and uses thereof
Guoping Zhao, Shanghai (China); Rui Heng Xu, Guangdong (China); Xinwei Wu, Guangdong (China); Changchun Tu, Jilin (China); Huai-Dong Song, Shanghai (China); Yixue Li, Shanghai (China); Jinlin Hou, Guangdong (China); Jun Xu, Guangdong (China); and Jun Min, Guangdong (China)
Assigned to Chinese National Human Genome Center at Shanghai, Shanghai (China)
Filed on Jul. 09, 2004, as Appl. No. 10/888,401.
Prior Publication US 2005/0112554 A1, May 26, 2005
Int. Cl. C12Q 1/70 (2006.01); C12Q 1/68 (2006.01); C07H 21/02 (2006.01)
U.S. Cl. 435—5  [435/6; 536/24.32; 536/23.72] 6 Claims
 
1. A method for detecting an early-stage SARS coronavirus comprising:
providing a sample suspected of containing the early stage SARS coronavirus; and
identifying the presence of at least one early-stage-specific molecular marker in a viral RNA, using a protocol comprising preparing coronaviral RNA from the sample, and obtaining cDNA using reverse transcription,
wherein the early-stage-specific molecular marker is selected from the group consisting of: an insertion early-stage-specific molecular marker, a deletion early-stage-specific molecular marker, and at least one single nucleotide variation (SNV) early-stage-specific molecular marker, wherein the SNV early-stage-specific molecular marker is C at position 4220.