US 7,320,889 B2 | ||
Cell cultivation device and method | ||
Wolfgang Kahlert, Körle (Germany); Bernd-Ulrich Wilhelm, Petershagen (Germany); Rainer Salzmann, Melsungen (Germany); and Wolfgang Rietschel, Söhrewald (Germany) | ||
Assigned to Sartorius Stedim Biotech GmbH, Goettingen (Germany) | ||
Filed on May 07, 2004, as Appl. No. 10/841,029. | ||
Claims priority of application No. 103 22 054 (DE), filed on May 15, 2003. | ||
Prior Publication US 2004/0229348 A1, Nov. 18, 2004 | ||
Int. Cl. C12M 1/08 (2006.01) |
U.S. Cl. 435—295.3 [435/286.5; 435/286.6; 435/297.2; 435/299.1] | 12 Claims |
1. A cell cultivation device comprising:
(a) a reactor vessel with a lid;
(b) a matrix-containing chamber within said reactor vessel, said matrix-containing chamber comprising a cylinder having a
base and an upper cap separate from said lid, a liquid-permeable upper plate and a liquid-permeable lower plate both within
said cylinder, a liquid-permeable matrix between said upper plate and said lower plate, whereby an antechamber is formed by
said upper plate, the wall of said cylinder and said upper cap;
(c) a cell culture chamber within said reactor vessel and surrounding said cylinder;
(d) an upper liquid level sensor and a lower liquid level sensor within said cell culture chamber; and
(e) at least two hydraulic fittings through said upper cap and extending into said antechamber so as to place said antechamber
in fluid communication with the exterior of said upper cap.
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