US 7,321,076 B2
Unactivated ungulate oocytes to produce a cloned ungulate by nuclear transfer
Keith Henry Stockman Campbell, Midlothian (United Kingdom); and Ian Wilmut, Midlothian (United Kingdom)
Assigned to Roslin Institute, Midlothian (United Kingdom)
Filed on Aug. 10, 2004, as Appl. No. 10/914,161.
Application 09/658862 is a division of application No. 08/803165, filed on Feb. 19, 1997, granted, now 6,252,133.
Application 10/914161 is a continuation of application No. 09/658862, filed on Sep. 08, 2000.
Application 08/803165 is a continuation of application No. PCT/GB96/02098, filed on Aug. 30, 1996.
Claims priority of application No. 9517779.6 (GB), filed on Aug. 31, 1995.
Prior Publication US 2005/0034181 A1, Feb. 10, 2005
This patent is subject to a terminal disclaimer.
Int. Cl. C12N 15/00 (2006.01); A01K 67/027 (2006.01)
U.S. Cl. 800—24  [800/8; 800/15; 800/16; 800/17] 54 Claims
OG exemplary drawing
 
1. A method of cloning an ungulate comprising:
(i) fusing a diploid, ungulate differentiated cell with an unactivated, enucleated metaphase II-arrested oocyte of the same species to reconstruct an embryo;
(ii) maintaining the reconstructed embryo without activation for a sufficient time to allow the reconstructed embryo to become capable of developing to term;
(iii) activating the resultant reconstructed embryo; and
(iv) transferring said reconstructed embryo to a host ungulate of the same species such that the reconstructed embryo develops to term.