US 7,320,868 B2
Leptin binding domain compositions and methods thereto
Arieh Gertler, 11 Hagefen Str, Rehovot 76345 (Israel); and Radha Krishna, 3711 Summer Cove Ct., Friendswood, Tex. 77546 (US)
Filed on Mar. 19, 2004, as Appl. No. 10/803,459.
Prior Publication US 2005/0209137 A1, Sep. 22, 2005
Int. Cl. G01N 33/53 (2006.01); G01N 33/566 (2006.01); C12Q 1/00 (2006.01); C07K 17/00 (2006.01); C07K 16/00 (2006.01); C12N 5/06 (2006.01)
U.S. Cl. 435—7.1  [435/4; 436/501; 530/350; 530/387.1] 12 Claims
 
1. A method for detecting a level of free leptin in a sample from an individual, comprising:
contacting the sample with a chicken leptin receptor binding domain of SEQ ID NO:8 for a time sufficient to allow binding between the free leptin and the leptin receptor binding domain to form a bound complex, wherein said receptor binding domain is bound to a solid phase;
washing the solid phase with a first wash buffer;
contacting the solid phase with an antibody having binding specificity to leptin, wherein said antibody is coupled with a detectable label;
washing the solid phase with a second wash buffer; and
detecting said label remaining with said solid phase, thus detecting the level of free leptin in the sample.