US 7,320,887 B2 | ||
Alkaline protease variants | ||
Beatrix Kottwitz, Erkrath (Germany); Karl-Heinz Maurer, Erkrath (Germany); and Roland Breves, Mettmann (Germany) | ||
Assigned to Henkel Kommanditgesellschaft auf Aktien, Duesseldorf (Germany) | ||
Filed on Apr. 30, 2004, as Appl. No. 10/836,959. | ||
Application 10/836959 is a continuation of application No. PCT/EP02/11725, filed on Oct. 19, 2002. | ||
Claims priority of application No. 101 53 792 (DE), filed on Oct. 31, 2001. | ||
Prior Publication US 2005/0003985 A1, Jan. 06, 2005 | ||
Int. Cl. C12N 9/54 (2006.01); C12N 15/57 (2006.01); C12N 15/74 (2006.01); C11D 3/386 (2006.01) |
U.S. Cl. 435—221 [435/69.1; 435/252.3; 435/320.1; 510/300] | 19 Claims |
1. A modified alkaline protease comprising:
a substitution of a threonine for the residue at the position corresponding to position 3 of the mature protease of SEQ ID
NO:2;
a substitution of an isoleucine for the residue at the position corresponding to position 4 of the mature protease of SEQ
ID NO:2;
a substitution of an alanine, a threonine, or an arginine for the residue at the position corresponding to position 61 of
the mature protease of SEQ ID NO:2; and
a substitution of an isoleucine for the residue at the position corresponding to position 199 of the mature protease of SEQ
ID NO:2,
wherein the modified alkaline protease is a mature protease comprising the gaps in amino acid sequence relative to the amino
acid sequences of subtilisin BPN' and subtilisin Carlsberg depicted in FIG. 1.
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