US 7,320,784 B1
Immobilized labeling compounds and methods
Alfred Pollak, Lujbjana (Slovenia); David Roe, Ontario (Canada); Linda Fung Lu, Scarborough (Canada); John Thornback, London (United Kingdom); and Catherine M. Pollock, Toronto (Canada)
Assigned to Bracco Imaging S.p.A., Milan (Italy)
Appl. No. 9/913,401
PCT Filed Aug. 03, 1999, PCT No. PCT/CA99/00700
§ 371(c)(1), (2), (4) Date Jan. 16, 2002,
PCT Pub. No. WO00/48639, PCT Pub. Date Aug. 24, 2000.
Int. Cl. A61K 49/00 (2006.01)
U.S. Cl. 424—9.1  [424/1.11; 424/1.37; 424/1.65; 424/9.3; 424/9.4; 424/9.5; 534/14] 39 Claims
 
1. A composition for generating a complex-forming metal ion labeled conjugate, the composition comprising:
(a) a metal support surface which is made of gold, silver or copper, or which is a substrate that is coated with gold, silver or copper, said substrate selected from the group consisting of inorganic silicate glass, alkylamino functionalized controlled-pore glass, silica, alumina beads, organic polystyrene, polyacrylamide, Sephadex, and agarose; and
(b) a conjugate releasably bound to the support surface, the conjugate comprising a ligand and a targeting molecule;
wherein the conjugate coordinates with a complex-forming metal ion so that the labeled conjugate is released from the support surface.