US 7,321,077 B2
Repressor-mediated regulation system for control of gene expression in plants
Abdelali Hannoufa, Saskatoon (Canada); Dwayne Hegedus, Saskatoon (Canada); and Nicholas Bate, Urbandale, Iowa (US)
Assigned to Her Majesty the Queen in Right of Canada as Represented by the Minister of Agriculture and Agri-food, Saskatchewan (Canada)
Filed on Nov. 21, 2003, as Appl. No. 10/719,996.
Application 10/719996 is a continuation of application No. PCT/CA02/00740, filed on May 23, 2002.
Claims priority of provisional application 60/292973, filed on May 23, 2001.
Prior Publication US 2004/0224412 A1, Nov. 11, 2004
Int. Cl. C12N 15/63 (2006.01); C12N 15/31 (2006.01); C12N 15/82 (2006.01)
U.S. Cl. 800—288  [800/278; 800/295; 800/298; 435/440; 435/468; 536/23.1; 536/23.7; 536/24.1] 5 Claims
 
1. A method for selectively controlling the transcription of a gene of interest in a plant, comprising:
i) producing a first plant comprising a first genetic construct, said first genetic construct comprising a first regulatory region operatively linked to a gene of interest and one, or more than one, ROS operator sequence capable of controlling the activity of said first regulatory region, the ROS operator sequence comprising a sequence selected from the group consisting of SEQ ID NO: 20, an inverted repeat of SEQ ID NO: 20, and a combination thereof;
ii) producing a second plant comprising a second genetic construct, said second genetic construct comprising a second regulatory region in operative association with a nucleic acid molecule, encoding a ROS repressor selected from the group consisting of Agrobacterium ROS and Rhizobium ROS optimized for expression in a plant, said ROS repressor exhibiting both ROS operator binding activity and ROS repressor activity and;
iii) crossing said first plant and said second plant to obtain progeny, said progeny comprising both said first genetic construct and said second genetic construct, and characterized in that the expression of said second genetic construct represses expression of said first genetic construct.