US 7,321,829 B2 | ||
Method for the identification and/or the quantification of a target compound obtained from a biological sample upon chips | ||
José Remacle, Malonne (Belgium); Joseph Demarteau, Namur (Belgium); Nathalie Zammatteo, Jambes (Belgium); Isabelle Alexandre, Lesve (Belgium); Sandrine Hamels, Loverval (Belgium); Yves Houbion, Floreffe (Belgium); and Francoise De Longueville, Jambes (Belgium) | ||
Assigned to Eppendorf Array Technologies (E.A.T.), Namur (Belgium) | ||
Filed on May 19, 2000, as Appl. No. 9/574,626. | ||
Claims priority of application No. 99870106 (EP), filed on May 19, 1999; and application No. 00870025 (EP), filed on Feb. 18, 2000. | ||
Prior Publication US 2003/0124522 A1, Jul. 03, 2003 | ||
Int. Cl. G01N 33/48 (2006.01); G01N 25/20 (2006.01); C12Q 1/68 (2006.01); C12Q 33/567 (2006.01); C12Q 1/42 (2006.01) |
U.S. Cl. 702—19 [435/6; 435/7.21; 435/7.9; 435/21; 422/50; 702/20] | 32 Claims |
1. A method for the identification and/or the quantification of a target compound obtained from a sample, comprising the steps
of:
putting into contact the target compound with a capture molecule in order to allow a specific binding between said target
compound with said capture molecule, said capture molecule being fixed upon a surface of a solid support according to an array
comprising discrete regions with a density of at least 20 of said discrete regions per cm2, each of said discrete regions being fixed with one species of capture molecules;
performing a catalytic reduction of a metal present in solution leading to formation of a metallic precipitate in one or more
of said discrete regions;
determining the possible presence and quantification of precipitate(s) in said discrete region(s); and correlating the presence
and quantification of the precipitate(s) at the discrete region(s) with the identification and/or a quantification of said
target compound.
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