US 7,320,862 B2 | ||
Microfluidic extraction method | ||
Cord F. Stähler, Gässelweg 15, 69469 Weinheim (Germany); Peer F. Stähler, Obere Clignetstrasse 14, 68167 Mannheim (Germany); and Manfred Müller, Mannheimerstrasse 11, 69198 Schriesheim (Germany) | ||
Appl. No. 10/492,014 PCT Filed Oct. 10, 2002, PCT No. PCT/EP02/11383 § 371(c)(1), (2), (4) Date Oct. 13, 2004, PCT Pub. No. WO03/031965, PCT Pub. Date Apr. 17, 2003. |
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Claims priority of application No. 101 49 947 (DE), filed on Oct. 10, 2001. | ||
Prior Publication US 2005/0040043 A1, Feb. 24, 2005 | ||
Int. Cl. C12Q 1/68 (2006.01); G01N 33/53 (2006.01); C07H 21/02 (2006.01); C07H 21/04 (2006.01) |
U.S. Cl. 435—6 [435/7.1; 536/23.1; 536/24.3] | 30 Claims |
1. A method for selectively isolating, in parallel, a plurality species of target molecules from a sample, comprising the
steps:
(a) providing a microfluidic carrier having an array of a plurality of different receptors which are immobilized on or in
the carrier wherein each different receptor is immobilized at a different position,
(b) directing a sample comprising target molecules to be selectively isolated in parallel through said microfluidic carrier
under conditions under which said target molecules can specifically bind to the receptors immobilized on the carrier,
(c) removing unbound material of the sample from the carrier, and
(d) isolating, in parallel, the target molecules bound to the carrier by selective elution of said species of target molecules.
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