US 7,321,791 B2 | ||
Spectral imaging of deep tissue | ||
Richard Levenson, Brighton, Mass. (US); and Paul J. Cronin, Charlestown, Mass. (US) | ||
Assigned to Cambridge Research and Instrumentation, Inc., Woburn, Mass. (US) | ||
Filed on Sep. 23, 2003, as Appl. No. 10/669,101. | ||
Prior Publication US 2005/0065440 A1, Mar. 24, 2005 | ||
Int. Cl. A61B 6/00 (2006.01) |
U.S. Cl. 600—476 [600/310; 600/473; 600/475; 356/432] | 34 Claims |
1. A method comprising:
illuminating a sample to cause the sample to emit radiation, wherein the sample is a living mammal comprising deep tissue
supporting a target compound, wherein the emitted radiation comprises emission from the target compound and emission from
one or more other components in the sample, wherein the emission from the other components of the sample comprises autofluorescence
from one or more layers of tissue in the sample different from a layer of tissue comprising the deep tissue, and wherein the
deep tissue is subdermal tissue;
spectrally filtering the emitted radiation with each of at least four different spectral weighting functions;
storing an image of the spectrally filtered radiation for each of the spectral weighting functions; and
processing the stored images to construct a deep tissue image of the sample in which signal from the other compounds is reduced
relative to signal from the target compound, wherein processing the stored images comprises constructing the deep tissue image
based on a weighted superposition of information in the stored images and at least one emission spectrum for the other components
in the sample.
|