US 7,320,893 B2
Lipoprotein fingerprinting methods using metal ion chelate salts
Ronald D Macfarlane, Temple, Tex. (US); Brian D Hosken, Bryan, Tex. (US); Zachlyn N Farwig, College Station, Tex. (US); Irma L Espinosa, College Station, Tex. (US); Christine L Myers, Bryan, Tex. (US); and Steven L Cockrill, College Station, Tex. (US)
Assigned to The Texas A&M University System, College Station, Tex. (US)
Filed on Mar. 08, 2004, as Appl. No. 10/795,237.
Application 10/795237 is a continuation of application No. 10/262163, filed on Oct. 01, 2002, granted, now 6,753,185.
Claims priority of provisional application 60/326236, filed on Oct. 01, 2001.
Prior Publication US 2004/0171078 A1, Sep. 02, 2004
This patent is subject to a terminal disclaimer.
Int. Cl. G01N 33/92 (2006.01)
U.S. Cl. 436—71  [436/512; 436/536; 436/538; 436/539; 435/7.1] 33 Claims
OG exemplary drawing
 
1. A method of obtaining the lipoprotein profile of a sample, the method comprising:
contacting the sample, a metal ion chelate complex density gradient forming agent, and a dye to produce an unseparated mixture, wherein the sample contains lipoproteins;
subjecting the unseparated mixture to a centrifuge force field under conditions suitable to produce a separated mixture, wherein the separated mixture comprises multiple lipoprotein fractions; and
visualizing by photographing or digital imaging the multiple lipoprotein fractions to obtain a lipoprotein profile.