US 7,321,831 B2 | ||
Nucleic acid fragment-fixed electrode and its use | ||
Yoshihiko Makino, Saitama (Japan); and Yoshihiko Abe, Saitama (Japan) | ||
Assigned to Fujifilm Corporation, Tokyo (Japan) | ||
Filed on Aug. 30, 2002, as Appl. No. 10/231,242. | ||
Claims priority of application No. 2001-265482 (JP), filed on Sep. 03, 2001. | ||
Prior Publication US 2003/0152958 A1, Aug. 14, 2003 | ||
Int. Cl. G01N 33/48 (2006.01); C12Q 1/00 (2006.01); C12Q 1/68 (2006.01); G01N 33/00 (2006.01); G06F 19/00 (2006.01) |
U.S. Cl. 702—19 [435/4; 435/6; 702/20; 702/27] | 14 Claims |
1. An electrode assembly, comprising:
an electrode;
a multi-component self-assembled monolayer of two or more different components formed on the electrode; and
a nucleic acid fragment fixed on the surface of the monolayer by a covalent bond via a bifunctional linking molecule, wherein
said bifunctional linking molecules does not comprise a nucleic acid, and
wherein the monolayer is composed of at least two components represented by the following formula (1) with the type of Jn being different:
qn-Rn-Jn (1)
wherein qn represents a group which is chemically bound to or adsorbed on the electrode, Rn represents a linking group, Jn represents different functional groups of n types, and n represents an integer of 2 or more, and in the components of formula
(1) of n types, qn and Rn may be identical or different from each other, and Jn are different from each other;wherein the bifunctional linking molecule is represented by the following formula (2):
X1-L1-X2 (2)
wherein X1 represents a group which forms a covalent bond with at least one type of the functional group Jn in the above formula (1), X2 represents a group which forms a covalent bond with a functional group Z in the following formula (3), and L1 represents a linking group; and
wherein the nucleic acid fragment is represented by the following formula (3):
Z-L2-Nc (3)
wherein Z represents a group which forms a covalent bond with the group X2 in the above formula (2), Nc represents a nucleic acid fragment, and L2 represents a linking group.
|