US 7,321,832 B2
Method of designing probe from polynucleotide group comprising plurality of polynucleotides
Tae-jin Ahn, Seoul (Korea, Republic of)
Assigned to Samsung Electronics Co., Ltd., (Korea, Republic of)
Filed on Oct. 26, 2005, as Appl. No. 11/259,730.
Claims priority of application No. 10-2004-0085798 (KR), filed on Oct. 26, 2004.
Prior Publication US 2006/0110757 A1, May 25, 2006
Int. Cl. G01N 33/48 (2006.01); C12Q 1/68 (2006.01); C07H 21/00 (2006.01)
U.S. Cl. 702—19  [435/6; 536/25.3] 6 Claims
 
1. A method of designing a probe from a polynucleotide group comprising a plurality of polynucleotides, the method comprising:
providing a sequence for each polynucleotide in a plurality of polynucleotides;
forming a polynucleotide group consisting of all the sequences;
producing polynucleotide fragments having a certain length from each of the sequences of the polynucleotide group;
obtaining a sequence for each polynucleotide fragment;
providing, for each polynucleotide fragment, a sequence specific identification number using the sequence for the polynucleotide fragment;
comparing the sequence specific identification numbers of the polynucleotide fragments to each other to select a probe; and
displaying the selected probe;
wherein the sequence specific identification number is calculated by
providing bases of nucleotides A, T, G, and C in the sequence of the polynucleotide fragment with values of 0, 4(L−i)/4, 4(L−i)/2, and 4(L−i)×(3/4), respectively, where L is the length of the polynucleotide fragment and i sequentially increases from i=0 at a 5′-end nucleotide of the polynucleotide to i=L−1 at a 3′-end nucleotide of the polynucleotide fragment;
adding the provided values for each base in the sequence and values from 41 to obtain a sum; and
providing the sum as the sequence specific identification number of the polynucleotide fragment.