US 7,320,865 B2 | ||
Detection of nucleic acid sequence differences using coupled ligase detection and polymerase chain reactions | ||
Francis Barany, New York, N.Y. (US); Matthew Lubin, Rye Brook, N.Y. (US); George Barany, Falcon Heights, Minn. (US); and Robert P. Hammer, Baton Rouge, La. (US) | ||
Assigned to Cornell Research Foundation, Inc., Ithaca, N.Y. (US); Regents of the University of Minnesota, Minneapolis, Minn. (US); and Board of Supervisors of Louisiana State University and Agricultural and Mechanical College, Baton Rouge, La. (US) | ||
Filed on Oct. 31, 2006, as Appl. No. 11/590,384. | ||
Application 09/440523 is a division of application No. 08/864473, filed on May 28, 1997, granted, now 6,027,889. | ||
Application 11/590384 is a continuation of application No. 11/229366, filed on Sep. 16, 2005. | ||
Application 11/229366 is a continuation of application No. 10/843720, filed on May 12, 2004, granted, now 7,166,434, filed on Jan. 23, 2007. | ||
Application 10/843720 is a continuation of application No. 09/918156, filed on Jul. 30, 2001, granted, now 6,797,470. | ||
Application 09/918156 is a continuation of application No. 09/440523, filed on Nov. 15, 1999, granted, now 6,268,148, filed on Jul. 31, 2001. | ||
Claims priority of provisional application 60/018532, filed on May 29, 1996. | ||
Prior Publication US 2007/0054306 A1, Mar. 08, 2007 | ||
This patent is subject to a terminal disclaimer. | ||
Int. Cl. C12Q 1/68 (2006.01); C12P 19/34 (2006.01) |
U.S. Cl. 435—6 [435/91.2] | 9 Claims |
1. A method for identifying a target nucleotide sequence comprising:
forming a ligation product on a target nucleotide sequence in a ligation detection reaction mixture, wherein the ligation
product comprises an upstream primer portion and a downstream primer portion, wherein the upstream primer portion and the
downstream primer portion are not complementary with the target nucleotide sequence;
amplifying the ligation product to form an amplified ligation product in a polymerase chain reaction (PCR) mixture, wherein
the PCR mixture comprises an upstream primer and a downstream primer, wherein the upstream primer contains the same sequence
as the upstream primer portion of the ligation product, and wherein the downstream primer is complementary to the downstream
primer portion of the ligation product; and
detecting the amplified ligation product to identify the target nucleotide sequence.
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