US 7,320,868 B2 | ||
Leptin binding domain compositions and methods thereto | ||
Arieh Gertler, 11 Hagefen Str, Rehovot 76345 (Israel); and Radha Krishna, 3711 Summer Cove Ct., Friendswood, Tex. 77546 (US) | ||
Filed on Mar. 19, 2004, as Appl. No. 10/803,459. | ||
Prior Publication US 2005/0209137 A1, Sep. 22, 2005 | ||
Int. Cl. G01N 33/53 (2006.01); G01N 33/566 (2006.01); C12Q 1/00 (2006.01); C07K 17/00 (2006.01); C07K 16/00 (2006.01); C12N 5/06 (2006.01) |
U.S. Cl. 435—7.1 [435/4; 436/501; 530/350; 530/387.1] | 12 Claims |
1. A method for detecting a level of free leptin in a sample from an individual, comprising:
contacting the sample with a chicken leptin receptor binding domain of SEQ ID NO:8 for a time sufficient to allow binding
between the free leptin and the leptin receptor binding domain to form a bound complex, wherein said receptor binding domain
is bound to a solid phase;
washing the solid phase with a first wash buffer;
contacting the solid phase with an antibody having binding specificity to leptin, wherein said antibody is coupled with a
detectable label;
washing the solid phase with a second wash buffer; and
detecting said label remaining with said solid phase, thus detecting the level of free leptin in the sample.
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