US 7,321,032 B2
Method of detecting Bifidobacterium infantis
Kazuyoshi Namba, Zama (Japan); Michiko Hatano, Zama (Japan); Tomoko Yaeshima, Zama (Japan); Norio Ishibashi, Zama (Japan); and Yoshitaka Tamura, Zama (Japan)
Assigned to Morinaga Milk Industry Co., Ltd., Minato-ku, Tokyo (Japan)
Appl. No. 10/543,491
PCT Filed Aug. 12, 2004, PCT No. PCT/JP2004/011609
§ 371(c)(1), (2), (4) Date Jul. 26, 2005,
PCT Pub. No. WO2005/080563, PCT Pub. Date Sep. 01, 2005.
Claims priority of application No. 2004-047720 (JP), filed on Feb. 24, 2004.
Prior Publication US 2006/0281084 A1, Dec. 14, 2006
Int. Cl. C07H 21/04 (2006.01); C12P 19/34 (2006.01); G01N 31/22 (2006.01)
U.S. Cl. 536—24.33  [435/91.2; 422/61] 6 Claims
 
1. PCR primers for detecting Bifidobacterium infantis comprising:
a first oligonucleotide consisting of a sequence of 20 or more continuous nucleotides selected from a nucleotide sequence of SEQ ID NO: 1; and
a second oligonucleotide consisting of a nucleotide sequence of SEQ ID NO: 2 or a nucleotide sequence of SEQ ID NO: 2 in which 0 to 7 nucleotides have been deleted from its 5′-terminal side.