US 7,321,075 B2
Serial nuclear transfer of ungulate embryos
Keith Henry Stockman Campbell, Midlothian (United Kingdom); and Ian Wilmut, Midlothian (United Kingdom)
Assigned to Roslin Institute (Edinburgh), Midlothian (United Kingdom)
Filed on Sep. 05, 2002, as Appl. No. 10/234,854.
Application 10/234854 is a continuation of application No. 10/118337, filed on Apr. 09, 2002, abandoned.
Application 10/118337 is a continuation of application No. 09/989125, filed on Nov. 21, 2001.
Application 09/989125 is a continuation of application No. 09/650285, filed on Aug. 29, 2000, granted, now 6,525,243.
Application 09/989125 is a continuation of application No. 10/234854.
Application 10/234854 is a continuation of application No. 10/151304, filed on May 21, 2002, abandoned.
Application 10/151304 is a continuation of application No. 09/989128, filed on Nov. 21, 2001.
Application 09/989128 is a continuation of application No. 09/650285.
Application 09/989128 is a continuation of application No. 10/234854.
Application 10/234854 is a continuation of application No. 10/151305, filed on May 21, 2002, abandoned.
Application 10/151305 is a continuation of application No. 09/989126, filed on Nov. 21, 2001.
Application 09/989126 is a continuation of application No. 09/650285.
Application 09/650285 is a continuation of application No. 08/803165, filed on Feb. 19, 1997, granted, now 6,252,133.
Application 08/803165 is a continuation of application No. PCT/GB96/02098, filed on Aug. 30, 1996.
Claims priority of application No. 9517779.6 (GB), filed on Aug. 31, 1995.
Prior Publication US 2003/0106081 A1, Jun. 05, 2003
Int. Cl. C12N 15/00 (2006.01); C12N 5/00 (2006.01)
U.S. Cl. 800—24  [435/375; 435/377] 8 Claims
 
1. A method of serial nuclear transfer comprising:
(i) providing a transgenic reconstructed ungulate embryo comprising a nucleus from a cultured diploid ungulate differentiated cell, wherein said embryo is capable of cell division;
(ii) culturing said embryo;
(iii) splitting said embryo into cells; and
(iv) inserting the nuclei of said cells into enucleated oocytes of the same species to reconstruct viable embryos.