CPC G01N 33/6848 (2013.01) [G01N 30/72 (2013.01); H01J 49/0036 (2013.01)] | 15 Claims |
1. A method for determining oxidation profiles for at least one peptide or protein in a sample, comprising:
contacting the sample to a solid surface, wherein the solid surface comprises a hydrophobic group and the solid surface is included in a chromatography column;
washing the solid surface using a mobile phase to produce at least one eluent, wherein the eluent comprises the at least one peptide or protein;
determining the oxidation profile of the at least one peptide or protein using a mass spectrometer; and
determining the oxidation variants in the sample;
wherein the mass spectrometer is coupled online to the chromatography column.
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