US 12,168,790 B2
Method of preparing toxin
Jae Young Kim, Seoul (KR); Jeong Sun Nam, Seoul (KR); Seungho Kim, Seoul (KR); Minjoong Kim, Gyeonggi-do (KR); Wonil Lee, Gyeonggi-do (KR); and Bum Jin Yun, Seoul (KR)
Assigned to JETEMA CO., LTD., Gangwon-do (KR)
Appl. No. 17/599,696
Filed by JETEMA CO., LTD., Gangwon-do (KR)
PCT Filed Mar. 12, 2020, PCT No. PCT/KR2020/003458
§ 371(c)(1), (2) Date Sep. 29, 2021,
PCT Pub. No. WO2020/204395, PCT Pub. Date Oct. 8, 2020.
Claims priority of application No. 10-2019-0036921 (KR), filed on Mar. 29, 2019.
Prior Publication US 2022/0195412 A1, Jun. 23, 2022
This patent is subject to a terminal disclaimer.
Int. Cl. C12P 21/02 (2006.01); A61K 38/48 (2006.01); C12N 9/52 (2006.01)
CPC C12N 9/52 (2013.01) [C12Y 304/24069 (2013.01)] 2 Claims
 
1. A method of preparing a botulinum toxin comprising:
(a) producing a botulinum toxin by culturing Clostridium botulinum type A in a medium composition for culturing Clostridium botulinum comprising 3% (w/v) of a potato peptone, 1% (w/v) of a yeast extract and 1% (w/v) glucose; and
(b) recovering the botulinum toxin within 40 hours after initiation of culture;
wherein the botulinum toxin has a higher yield than when phytone peptone is used as the peptone source.