US 12,168,805 B2
Methods and reagents for detecting piperaquine-resistant plasmodium falciparum malaria
Didier Menard, Le Gâvre (FR); Frédéric Ariey, Chevilly Larue (FR); Benoit Witkowski, Montauban (FR); Valentine Duru, Le Pre Saint Gervais (FR); Nimol Khim, Kandal (KH); Johann Beghain, Conflans Sainte-Honorine (FR); Benjamin Saint Pierre, Versailles (FR); and Eric Legrand, Le Blanc-Mesnil (FR)
Assigned to INSTITUT PASTEUR, Paris (FR); INSTITUT PASTEUR DU CAMBODGE, Phnom Penh (KH); and CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE, Paris (FR)
Appl. No. 16/324,140
Filed by INSTITUT PASTEUR, Paris (FR); INSTITUT PASTEUR DU CAMBODGE, Phnom Penh (KH); and CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE, Paris (FR)
PCT Filed Aug. 4, 2017, PCT No. PCT/IB2017/001125
§ 371(c)(1), (2) Date Feb. 8, 2019,
PCT Pub. No. WO2018/029532, PCT Pub. Date Feb. 15, 2018.
Claims priority of provisional application 62/373,179, filed on Aug. 10, 2016.
Prior Publication US 2019/0218623 A1, Jul. 18, 2019
Int. Cl. C12Q 1/68 (2018.01); C12Q 1/6827 (2018.01); C12Q 1/6893 (2018.01)
CPC C12Q 1/6893 (2013.01) [C12Q 1/6827 (2013.01); C12Q 2600/156 (2013.01); C12Q 2600/158 (2013.01); G01N 2333/445 (2013.01); Y02A 50/30 (2018.01)] 17 Claims
 
1. A method for in vitro genotyping and treatment of a Plasmodium resistant to piperaquine comprising:
extracting DNA from a sample containing a Plasmodium from a patient infected by a Plasmodium,
measuring the number of copies per genome of PfPM2 and PfPM3 genes, and
detecting the presence of at least 2 copies per genome of PfPM2 and PfPM3 genes of the Plasmodium resistant to piperaquine in the extracted DNA, and
treating the patient with an anti-malaria treatment without piperaquine if the patient is infected by a Plasmodium that has at least 2 copies per genome of PfPM2 and PfPM3 genes.