CPC A61K 35/34 (2013.01) [A61K 38/25 (2013.01); C12N 1/38 (2013.01); C12N 5/0657 (2013.01); C12N 5/0696 (2013.01); C12N 5/10 (2013.01); C12N 2501/415 (2013.01); C12N 2501/60 (2013.01); C12N 2501/85 (2013.01); C12N 2506/45 (2013.01)] | 4 Claims |
1. A method of obtaining a population of human cardiomyogenic precursor cells, the method comprising:
(a) differentiating human induced pluripotent stem cells (hiPSCs) in the presence of all of a glycogen synthase kinase 3 (GSK3) inhibitor, a Wnt inhibitor, and recombinant growth hormone releasing hormone or their synthetic analogs to obtain hiPSC-derived human cardiac precursor cells; and
(b) isolating a population of human cardiac precursor cells based on (i) expression of human growth hormone-releasing hormone receptor (GHRHR) using a GHRHR antibody and (ii) no expression of c-Kit to obtain a population consisting essentially of hiPSC-derived human cardiomyogenic precursor cells which express Nkx2.5 and Isl1.
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