CPC G01N 33/532 (2013.01) [C07K 14/70539 (2013.01); C12N 15/1037 (2013.01); C12N 15/1055 (2013.01); C12N 15/1065 (2013.01); C12N 15/1075 (2013.01); C12N 15/11 (2013.01); C12N 15/85 (2013.01); C12Q 1/6804 (2013.01); C12Q 1/6806 (2013.01); C12Q 1/6818 (2013.01); C12Q 1/6827 (2013.01); C12Q 1/6881 (2013.01); G01N 33/5032 (2013.01); G01N 33/505 (2013.01); G01N 33/5304 (2013.01); G01N 33/5306 (2013.01); G01N 33/5308 (2013.01); G01N 33/54306 (2013.01); G01N 33/54366 (2013.01); G01N 33/548 (2013.01); G01N 33/56977 (2013.01); G01N 33/58 (2013.01); C12N 2310/20 (2017.05); C12N 2320/10 (2013.01); C12Q 2537/164 (2013.01); C12Q 2563/179 (2013.01); C12Q 2563/185 (2013.01); C12Q 2565/1015 (2013.01); C40B 30/04 (2013.01); C40B 50/06 (2013.01); C40B 70/00 (2013.01)] | 22 Claims |
1. A mixture, comprising:
a nucleic acid comprising: (i) a first moiety, (ii) a nucleic acid sequence, and (ii) a poly-thymine (poly(T)) sequence, a poly-guanine (poly(G)) sequence, or an N-mer sequence;
a nucleic acid barcode comprising a first strand and a second strand, wherein said first strand comprises a second moiety and a barcode sequence, wherein said second strand comprises (1) a first sequence complementary to said barcode sequence and (2) an overhang comprising a second sequence complementary to said nucleic acid sequence; and
reagents configured to non-enzymatically ligate said first moiety to said second moiety.
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