US 12,169,271 B2
Method for evaluating measurement data from a light field microscope, and apparatus for light field microscopy
Daniel Schwedt, Jena (DE); and Tiemo Anhut, Jena (DE)
Assigned to Carl Zeiss Microscopy GmbH, Jena (DE)
Filed by Carl Zeiss Microscopy GmbH, Jena (DE)
Filed on Sep. 7, 2022, as Appl. No. 17/939,294.
Claims priority of application No. 10 2021 123 148.5 (DE), filed on Sep. 7, 2021.
Prior Publication US 2023/0070373 A1, Mar. 9, 2023
Int. Cl. G02B 21/36 (2006.01); G02B 21/00 (2006.01)
CPC G02B 21/367 (2013.01) [G02B 21/002 (2013.01)] 27 Claims
OG exemplary drawing
 
1. A method for evaluating measurement data from a light field microscope, comprising the following steps:
a) selecting at least one sample position in a sample to be analyzed;
b) recording multiple images of the sample at a sequence of recording times using a light field microscope, wherein for each image of the sample a set of partial images is being simultaneously recorded at different angles relative to the sample;
c) wherein positions corresponding to the selected sample positions are determined in the partial images of the recorded images;
d) extracting image signals from at least some of the partial images at the determined positions;
e) obtaining an integrated signal for a certain recording time by the image signals extracted for a certain position from partial images of this recording time being integrated to form the integrated signal; and
f) obtaining a time profile of the integrated signal by obtaining the integrated signal for a plurality of recording times.