CPC C12Q 1/689 (2013.01) [C12Q 1/6853 (2013.01); C12Q 1/686 (2013.01); C12Q 1/6869 (2013.01); G01N 33/52 (2013.01); C12Q 2600/156 (2013.01); G01N 2458/00 (2013.01); G01N 2469/00 (2013.01)] | 9 Claims |
1. A primer set for detection of Listeria monocytogenes, comprising any of the following primer sets for amplification of a partial region of lmo0084 gene or lmo2736 gene of Listeria monocytogenes:
(A-1) a set of a forward primer containing in its 3′-side the base sequence of SEQ ID NO:26 or a sequence which is the same as said base sequence except that not more than 4 bases are substituted at a genetic polymorphism site(s) in said base sequence, and a reverse primer containing in its 3′-side the base sequence of SEQ ID NO:30 or a sequence which is the same as said base sequence except that not more than 4 bases are substituted at a genetic polymorphism site(s) in said base sequence;
(A-2) a set of a forward primer containing in its 3′-side the base sequence of SEQ ID NO:26 or a sequence which is the same as said base sequence except that not more than 4 bases are substituted at a genetic polymorphism site(s) in said base sequence, and a reverse primer containing in its 3′-side the base sequence of SEQ ID NO:31 or a sequence which is the same as said base sequence except that not more than 4 bases are substituted at a genetic polymorphism site(s) in said base sequence;
(A-3) a set of a forward primer containing in its 3′-side the base sequence of SEQ ID NO:27 or a sequence which is the same as said base sequence except that not more than 4 bases are substituted at a genetic polymorphism site(s) in said base sequence, and a reverse primer containing in its 3′-side the base sequence of SEQ ID NO:30 or a sequence which is the same as said base sequence except that not more than 4 bases are substituted at a genetic polymorphism site(s) in said base sequence;
(A-4) a set of a forward primer containing in its 3′-side the base sequence of SEQ ID NO:27 or a sequence which is the same as said base sequence except that not more than 4 bases are substituted at a genetic polymorphism site(s) in said base sequence, and a reverse primer containing in its 3′-side the base sequence of SEQ ID NO:31 or a sequence which is the same as said base sequence except that not more than 4 bases are substituted at a genetic polymorphism site(s) in said base sequence;
(A-5) a set of a forward primer containing in its 3′-side the base sequence of SEQ ID NO:28 or a sequence which is the same as said base sequence except that not more than 4 bases are substituted at a genetic polymorphism site(s) in the base sequence, and a reverse primer containing in its 3′-side the base sequence of SEQ ID NO:30 or a sequence which is the same as said base sequence except that not more than 4 bases are substituted at a genetic polymorphism site(s) in said base sequence;
(A-6) a set of a forward primer containing in its 3′-side the base sequence of SEQ ID NO:28 or a sequence which is the same as said base sequence except that not more than 4 bases are substituted at a genetic polymorphism site(s) in said base sequence, and a reverse primer containing in its 3′-side the base sequence of SEQ ID NO:31 or a sequence which is the same as said base sequence except that not more than 4 bases are substituted at a genetic polymorphism site(s) in said base sequence;
(A-7) a set of a forward primer containing in its 3′-side the base sequence of SEQ ID NO:29 or a sequence which is the same as said base sequence except that not more than 4 bases are substituted at a genetic polymorphism site(s) in said base sequence, and a reverse primer containing in its 3′-side the base sequence of SEQ ID NO:30 or a sequence which is the same as said base sequence except that not more than 4 bases are substituted at a genetic polymorphism site(s) in said base sequence;
(A-8) a set of a forward primer containing in its 3′-side the base sequence of SEQ ID NO:29 or a sequence which is the same as said base sequence except that not more than 4 bases are substituted at a genetic polymorphism site(s) in said base sequence, and a reverse primer containing in its 3′-side the base sequence of SEQ ID NO:31 or a sequence which is the same as said base sequence except that not more than 4 bases are substituted at a genetic polymorphism site(s) in said base sequence;
(B-1) a set of a forward primer containing in its 3′-side the base sequence of SEQ ID NO:58 or a sequence which is the same as said base sequence except that not more than 4 bases are substituted at a genetic polymorphism site(s) in said base sequence, and a reverse primer containing in its 3′-side the base sequence of SEQ ID NO:59 or a sequence which is the same as said base sequence except that not more than 4 bases are substituted at a genetic polymorphism site(s) in said base sequence;
(C-1) a set of a forward primer containing in its 3′-side the base sequence of SEQ ID NO:32 or a sequence which is the same as said base sequence except that not more than 4 bases are substituted at a genetic polymorphism site(s) in said base sequence, and a reverse primer containing in its 3′-side the base sequence of SEQ ID NO:37 or a sequence which is the same as said base sequence except that not more than 4 bases are substituted at a genetic polymorphism site(s) in said base sequence;
(D-1) a set of a forward primer containing in its 3′-side the base sequence of SEQ ID NO:33 or a sequence which is the same as said base sequence except that not more than 4 bases are substituted at a genetic polymorphism site(s) in said base sequence, and a reverse primer containing in its 3′-side the base sequence of SEQ ID NO:38 or a sequence which is the same as said base sequence except that not more than 4 bases are substituted at a genetic polymorphism site(s) in said base sequence;
(E-1) a set of a forward primer containing in its 3′-side the base sequence of SEQ ID NO:34 or a sequence which is the same as said base sequence except that not more than 4 bases are substituted at a genetic polymorphism site(s) in the base sequence, and a reverse primer containing in its 3′-side the base sequence of SEQ ID NO:38 or a sequence which is the same as said base sequence except that not more than 4 bases are substituted at a genetic polymorphism site(s) in said base sequence;
(F-1) a set of a forward primer containing in its 3′-side the base sequence of SEQ ID NO:34 or a sequence which is the same as said base sequence except that not more than 4 bases are substituted at a genetic polymorphism site(s) in said base sequence, and a reverse primer containing in its 3′-side the base sequence of SEQ ID NO:39 or a sequence which is the same as said base sequence except that not more than 4 bases are substituted at a genetic polymorphism site(s) in said base sequence;
(G-1) a set of a forward primer containing in its 3′-side the base sequence of SEQ ID NO:34 or a sequence which is the same as said base sequence except that not more than 4 bases are substituted at a genetic polymorphism site(s) in said base sequence, and a reverse primer containing in its 3′-side the base sequence of SEQ ID NO:40 or a sequence which is the same as said base sequence except that not more than 4 bases are substituted at a genetic polymorphism site(s) in said base sequence;
(H-1) a set of a forward primer containing in its 3′-side the base sequence of SEQ ID NO:35 or a sequence which is the same as said base sequence except that not more than 4 bases are substituted at a genetic polymorphism site(s) in the base sequence, and a reverse primer containing in its 3′-side the base sequence of SEQ ID NO:39 or a sequence which is the same as said base sequence except that not more than 4 bases are substituted at a genetic polymorphism site(s) in said base sequence;
(I-1) a set of a forward primer containing in its 3′-side the base sequence of SEQ ID NO:35 or a sequence which is the same as said base sequence except that not more than 4 bases are substituted at a genetic polymorphism site(s) in said base sequence, and a reverse primer containing in its 3′-side the base sequence of SEQ ID NO:40 or a sequence which is the same as said base sequence except that not more than 4 bases are substituted at a genetic polymorphism site(s) in said base sequence;
(I-2) a set of a forward primer containing in its 3′-side the base sequence of SEQ ID NO:60 or a sequence which is the same as said base sequence except that not more than 4 bases are substituted at a genetic polymorphism site(s) in said base sequence, and a reverse primer containing in its 3′-side the base sequence of SEQ ID NO:61 or a sequence which is the same as said base sequence except that not more than 4 bases are substituted at a genetic polymorphism site(s) in said base sequence;
(I-3) a set of a forward primer containing in its 3′-side the base sequence of SEQ ID NO:62 or a sequence which is the same as said base sequence except that not more than 4 bases are substituted at a genetic polymorphism site(s) in said base sequence, and a reverse primer containing in its 3′-side the base sequence of SEQ ID NO:61 or a sequence which is the same as said base sequence except that not more than 4 bases are substituted at a genetic polymorphism site(s) in said base sequence;
(J-1) a set of a forward primer containing in its 3′-side the base sequence of SEQ ID NO:35 or a sequence which is the same as said base sequence except that not more than 4 bases are substituted at a genetic polymorphism site(s) in said base sequence, and a reverse primer containing in its 3′-side the base sequence of SEQ ID NO:41 or a sequence which is the same as said base sequence except that not more than 4 bases are substituted at a genetic polymorphism site(s) in said base sequence;
(K-1) a set of a forward primer containing in its 3′-side the base sequence of SEQ ID NO:36 or a sequence which is the same as said base sequence except that not more than 4 bases are substituted at a genetic polymorphism site(s) in said base sequence, and a reverse primer containing in its 3′-side the base sequence of SEQ ID NO:39 or a sequence which is the same as said base sequence except that not more than 4 bases are substituted at a genetic polymorphism site(s) in the base sequence;
(L-1) a set of a forward primer containing in its 3′-side the base sequence of SEQ ID NO:36 or a sequence which is the same as said base sequence except that not more than 4 bases are substituted at a genetic polymorphism site(s) in said base sequence, and a reverse primer containing in its 3′-side the base sequence of SEQ ID NO:40 or a sequence which is the same as said base sequence except that not more than 4 bases are substituted at a genetic polymorphism site(s) in said base sequence; and
(M-1) a set of a forward primer containing in its 3′-side the base sequence of SEQ ID NO:63 or a sequence which is the same as said base sequence except that not more than 4 bases are substituted at a genetic polymorphism site(s) in said base sequence, and a reverse primer containing in its 3′-side the base sequence of SEQ ID NO:64 or a sequence which is the same as said base sequence except that not more than 4 bases are substituted at a genetic polymorphism site(s) in said base sequence wherein the primer set further comprises a probe containing an oligonucleotide portion, wherein the ends of the oligonucleotide portion are modified with a fluorescent substance and a quencher substance, respectively.
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