CPC G01N 33/54306 (2013.01) [A61K 38/465 (2013.01); A61K 47/26 (2013.01); G01N 21/6428 (2013.01); G01N 30/7233 (2013.01); G01N 30/7266 (2013.01); G01N 33/6848 (2013.01); C12Y 301/01001 (2013.01)] | 29 Claims |
1. A method of identifying host cell protein (HCP) impurities in a sample containing at least one high-abundance protein, the method comprising:
contacting the sample with at least one probe capable of attaching to a HCP impurity, wherein the probe includes a warhead and a tag, and wherein the warhead is capable of binding to an active site of the probe-attached HCP impurity;
contacting the sample with a solid support, wherein the solid support comprises a ligand, and wherein the ligand is capable of binding to the tag; and
washing the solid support using a solution to provide an eluent to isolate the probe-attached HCP impurity.
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