CPC A61L 27/26 (2013.01) [A61L 27/225 (2013.01); A61L 27/507 (2013.01); A61L 27/54 (2013.01); A61L 31/046 (2013.01); A61L 33/0011 (2013.01); A61L 33/08 (2013.01); A61L 2300/236 (2013.01); A61L 2300/252 (2013.01); A61L 2300/254 (2013.01); A61L 2300/418 (2013.01); A61L 2300/42 (2013.01); A61L 2420/02 (2013.01); A61L 2430/06 (2013.01); A61L 2430/20 (2013.01)] | 11 Claims |
1. A method for producing a fibrin-based bioartificial and acellular planar construct, comprising:
provision of a fibrinogen-containing and cell-free composition;
provision of a thrombin-containing and cell-free composition;
application of the fibrinogen-containing cell-free and the thrombin-containing cell-free compositions to a mould as an applied layer of a mixture of compositions;
pressurization of the mixture of compositions applied to the mould at least until the fibrin formation is essentially completed; and
removal of the fibrin-based acellular bioartificial planar construct from the mould,
wherein pressure is applied in a planar manner to the compositions applied to the mould,
wherein the pressurization takes place at a relative pressure of at least 1 bar,
wherein the fibrin-based acellular bioartificial construct has at least a portion with a wall thickness of at least 1 mm, and
wherein the mould has through holes.
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