CPC C12P 21/005 (2013.01) [C12N 5/0043 (2013.01); C12N 2500/20 (2013.01); C12N 2500/33 (2013.01); C12N 2500/34 (2013.01); C12N 2511/00 (2013.01)] | 21 Claims |
1. A method for modifying the glycosylation profile of a recombinant glycoprotein produced in cell culture comprising:
culturing eukaryotic cells expressing the recombinant glycoprotein in a cell culture medium, wherein the cell culture medium is supplemented with fucose, manganese, and taurine,
wherein the glycosylation profile comprises a fucosylation profile and/or galactosylation profile, and
wherein the fucosylation profile and/or galactosylation profile of the produced recombinant glycoprotein is modified to better resemble the fucosylation profile and/or galactosylation profile_of a reference glycoprotein than when the eukaryotic cells expressing the recombinant glycoprotein are cultured without fucose, manganese, and taurine supplementation.
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