CPC B01J 19/0046 (2013.01) [C40B 50/14 (2013.01); B01J 2219/00596 (2013.01); B01J 2219/00626 (2013.01); B01J 2219/00653 (2013.01); B01J 2219/00659 (2013.01); B01J 2219/00673 (2013.01); B01J 2219/00689 (2013.01); B01J 2219/00713 (2013.01)] | 12 Claims |
1. A method of de novo nucleotide chain assembly comprising:
immobilizing a template-independent enzyme to an electrode platform comprising an electrode with a seeded inert surface functionalized with one or more links, the template-independent enzyme immobilized to the electrode platform via one or more of the links;
controlling, via direct electrical-field modulation, the immobilized template-independent enzyme to assemble a first nucleotide of a nucleotide chain by electrically modulating the electrode; and
controlling, via direct electrical-field modulation, the immobilized template-independent enzyme to assemble a next nucleotide of the nucleotide chain by electrically modulating the electrode;
wherein the assembly of the nucleotide chain is a de novo assembly of the nucleotide chain without using primer and without using template enzymes.
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