CPC C12N 9/14 (2013.01) [C12N 9/16 (2013.01); C12N 9/20 (2013.01); C12N 9/2414 (2013.01); C12N 9/2417 (2013.01); C12N 9/2428 (2013.01); C12N 9/2437 (2013.01); C12N 9/2457 (2013.01); C12N 9/50 (2013.01); C12P 7/06 (2013.01); C12P 19/14 (2013.01); C12Y 301/03008 (2013.01); C12Y 301/04 (2013.01); C12Y 301/04011 (2013.01); C12Y 302/01001 (2013.01); C12Y 302/01003 (2013.01); C12Y 302/01004 (2013.01); C12Y 302/01008 (2013.01); C12Y 302/01041 (2013.01); C12Y 304/21062 (2013.01); C12Y 304/23023 (2013.01); C12Y 304/24039 (2013.01); C12P 7/64 (2013.01); Y02E 50/10 (2013.01)] | 12 Claims |
1. A process of recovering oil, comprising
(a) converting a starch-containing material into dextrins with an alpha-amylase;
(b) saccharifying the dextrins using a carbohydrate source generating enzyme to form a sugar;
(c) fermenting the sugar in a fermentation medium into a fermentation product using a fermenting organism;
(d) recovering the fermentation product to form a whole stillage;
(e) separating the whole stillage into thin stillage and wet cake;
(e′) optionally concentrating the thin stillage into syrup;
(f) recovering oil from the thin stillage and/or optionally the syrup, wherein a protease and a phospholipase are present and/or added during steps (a) to (c);
wherein the protease present and/or added in steps (a) to (c) is a protease having the amino acid sequence set forth in SEQ ID NO: 9 or a protease having at least 85% identity to SEQ ID NO: 9 and having protease activity; and
wherein the phospholipase present and/or added in steps (a) to (c) is:
(i) the phospholipase shown in SEQ ID NO: 15 or one having a sequence identity thereto of at least 90% and having phospholipase activity;
(ii) the phospholipase shown in SEQ ID NO: 16 or one having a sequence identity thereto of at least 90% and having phospholipase activity; or
(iii) the phospholipase shown in SEQ ID NO: 17 or one having a sequence identity thereto of at least 90% and having phospholipase activity.
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