CPC A01N 1/0284 (2013.01) [A01N 1/0221 (2013.01); A01N 1/0247 (2013.01)] | 29 Claims |
1. A method for preserving a biological sample, the method comprising:
(a) perfusing the biological sample at a first perfusion rate with a first loading solution comprising one or more cryoprotective agent(s) at a hypothermic temperature between 0° C. and 12° C.;
(b) perfusing the biological sample at a second perfusion rate lower than the first perfusion rate with a second loading solution comprising a higher concentration of at least one of the one or more cryoprotective agent(s) than in the first loading solution at a hypothermic temperature;
(c) placing the perfused biological sample in a container;
(d) removing air from the container to avoid ice crystal formation in cells within the biological sample or the first and second loading solutions, and sealing the container; and
(e) cooling the biological sample in the container to a subzero temperature, thereby preserving the biological sample at a subzero temperature.
|