CPC C07K 16/18 (2013.01) [A61K 39/3955 (2013.01); A61K 39/39558 (2013.01); A61K 47/6803 (2017.08); A61K 47/68031 (2023.08); A61K 47/68033 (2023.08); A61P 35/00 (2018.01); C07K 16/30 (2013.01); C07K 16/3023 (2013.01); G01N 33/574 (2013.01); A61K 38/07 (2013.01); A61K 2039/505 (2013.01); A61K 47/6817 (2017.08); A61K 47/6843 (2017.08); A61K 47/6849 (2017.08); A61K 47/6891 (2017.08); A61K 49/0008 (2013.01); C07K 14/47 (2013.01); C07K 16/005 (2013.01); C07K 16/28 (2013.01); C07K 16/2866 (2013.01); C07K 2317/21 (2013.01); C07K 2317/35 (2013.01); C07K 2317/55 (2013.01); C07K 2317/622 (2013.01); C07K 2317/73 (2013.01); C07K 2317/77 (2013.01); C12N 15/1037 (2013.01); C12N 15/64 (2013.01); C12N 2810/00 (2013.01); C12Q 1/6869 (2013.01); G01N 33/5011 (2013.01); G01N 33/57423 (2013.01)] | 18 Claims |
1. A method for selecting antibody binding fragments that home to a tumor in vivo in a mammalian host, the method comprising:
a) screening a phage display library against at least one known tumor target to generate an enriched multivalent phage display library;
b) introducing phage particles from the enriched multivalent phage display library into a live mammalian host, wherein the mammalian host bears at least one tumor;
c) allowing the phage particles to home to the at least one tumor;
d) harvesting tumor tissue from the at least one tumor in the mammalian host;
e) extracting DNA from the tumor tissue;
f) isolating from the extracted DNA phage particles displaying antibody binding fragments that have bound to the tumor tissue in vivo; and
g) identifying the antibody binding fragments in the isolated phage particles.
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