CPC C12N 15/86 (2013.01) [A61K 9/0019 (2013.01); A61P 25/28 (2018.01); C07K 14/47 (2013.01); C12N 15/1137 (2013.01); C12N 15/64 (2013.01); C12Y 115/01001 (2013.01)] | 54 Claims |
1. A method of manufacturing an AAV viral vector, comprising:
a. acidifying and clarifying a cell lysate from cells expressing the AAV viral vector;
b. purifying the product of (a) using cation exchange chromatography (CEX);
c. filtering the product of (b) using a first tangential flow filtration (TFF) step;
d. ultracentrifuging the product of (c) in a cesium chloride (CsCl) buffer; and
e. collecting the AAV viral vector from the product of (d);
wherein a composition comprising the AAV viral vector from (d) comprises less than 2×105 pg/mL residual host cell DNA per 1×1013 vg/mL.
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