CPC C12N 9/1252 (2013.01) [C12Q 1/6806 (2013.01); C12Q 1/6869 (2013.01); C12Y 207/07007 (2013.01)] | 12 Claims |
1. A protein, which is A) or B) as follows:
A) a protein that has DNA polymerase activity obtained by substituting the following of wild-type KOD DNA polymerase:
the valine at position 389 with isoleucine; the valine at position 589 with histidine; the threonine at position 676 with lysine; the valine at position 680 with methionine; the serine at position 383 with threonine; the tyrosine at position 384 with phenylalanine;
B) a protein derived from A) that has DNA polymerase activity by adding a tag sequence to the end of the amino acid sequence of the protein shown in A);
wherein the amino acid sequence of wild-type KOD DNA polymerase is shown in SEQ ID NO: 1.
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