US 12,168,781 B2
Production method for T cells or NK cells, medium for culturing T cells or NK cells, method for culturing T cells or NK cells, method for maintaining undifferentiated state of undifferentiated T cells, and growth-accelerating agent for T cells or NK cells
Shinichiro Takayanagi, Tokyo (JP); Atsushi Kunisato, Tokyo (JP); Sayaka Chuganji, Tokyo (JP); Ken Fukumoto, Tokyo (JP); Kazuki Nakazono, Tokyo (JP); Shin Kaneko, Kyoto (JP); and Yohei Kawai, Kyoto (JP)
Assigned to KIRIN HOLDINGS KABUSHIKI KAISHA, Tokyo (JP); and Kyoto University, Kyoto (JP)
Appl. No. 17/311,001
Filed by KIRIN HOLDINGS KABUSHIKI KAISHA, Tokyo (JP); and Kyoto University, Kyoto (JP)
PCT Filed Dec. 6, 2019, PCT No. PCT/JP2019/047808
§ 371(c)(1), (2) Date Jun. 4, 2021,
PCT Pub. No. WO2020/116606, PCT Pub. Date Jun. 11, 2020.
Claims priority of application No. 2018-229478 (JP), filed on Dec. 6, 2018.
Prior Publication US 2022/0017867 A1, Jan. 20, 2022
Int. Cl. C12N 5/0783 (2010.01); C07D 215/00 (2006.01); C07D 217/22 (2006.01); C07D 471/18 (2006.01)
CPC C12N 5/0646 (2013.01) [C07D 215/00 (2013.01); C07D 217/22 (2013.01); C07D 471/18 (2013.01); C12N 5/0637 (2013.01); C12N 2501/48 (2013.01); C12N 2501/999 (2013.01)] 13 Claims
 
1. A method for producing T cells or NK cells, comprising culturing T cells or NK cells in a culture medium containing a bisbenzylisoquinoline alkaloid represented by the following formula (X-1) or formula (X-2) or a compound resulting from cleavage of one ether bond thereof or a pharmaceutically acceptable salt thereof:

OG Complex Work Unit Chemistry
R1 and R′1 are each independently H or straight-chain or branched-chain alkyl having 1 to 10 carbon atoms,
R2, R′2, R3, R′3, R4, R′4, R5, and R′5 are each independently H, acyl, straight-chain or branched-chain alkyl having 1 to 10 carbon atoms, and the alkyl may be interrupted by an O, N, or S heteroatom, or R2 and R3, R4 and R5, R′2 and R′3, or R′4 and R′5 together represent O or S,
R6, R′6, R7, and R′7 are each independently H, acyl, or straight-chain or branched-chain alkyl having 1 to 10 carbon atoms, and the alkyl may be interrupted by an O, N, or S heteroatom, or R6 and R7 or R′6 and R′7 together represent O or S, and
X1, X2, X3, and X4 may be the same or different, and are each independently H, hydroxy, straight-chain or branched-chain alkyl having 1 to 10 carbon atoms, alkoxy, acyloxy, or sulfonyloxy, and each may further have a substituent, n1 and n3 are each independently an integer of 0 to 3, n2 and n4 are each independently an integer of 0 to 4, and these may be linked to each other to form a ring, wherein the culturing period is 50 days or more.