CPC C12N 9/22 (2013.01) [C12N 15/102 (2013.01); C12N 15/11 (2013.01); G16B 15/00 (2019.02); G16B 15/20 (2019.02); G16B 15/30 (2019.02); C07K 2299/00 (2013.01); C07K 2319/09 (2013.01); C07K 2319/095 (2013.01); C07K 2319/20 (2013.01); C07K 2319/70 (2013.01); C12N 2310/20 (2017.05); C12N 2800/80 (2013.01)] | 25 Claims |
1. A non-naturally occurring and engineered SaCas9 protein comprising at least one modification or mutation compared to a wild-type SaCas9 protein, wherein the modification or mutation comprises mutation of at least one amino acid of the SaCas9 protein that interacts with sugar-phosphate backbone of an sgRNA or sugar-phosphate backbone of a target DNA strand, wherein the at least one amino acid comprises Thr238, Tyr239, Lys248, Tyr256, Arg314, Asn394, Gln414, Tyr211, Trp229, Tyr230, Gly235, Arg245, Gly391, Thr392, Asn419, Leu446, Tyr651, or Arg654.
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