CPC C12Q 1/686 (2013.01) [B01L 3/502715 (2013.01); B01L 3/527 (2013.01); B01L 7/52 (2013.01); B01L 3/5029 (2013.01); B01L 2200/025 (2013.01); B01L 2200/028 (2013.01); B01L 2200/04 (2013.01); B01L 2200/0647 (2013.01); B01L 2200/0684 (2013.01); B01L 2200/143 (2013.01); B01L 2300/023 (2013.01); B01L 2300/027 (2013.01); B01L 2300/0672 (2013.01); B01L 2300/123 (2013.01); B01L 2400/0421 (2013.01); B01L 2400/043 (2013.01); B01L 2400/0478 (2013.01); B01L 2400/0616 (2013.01); G01N 27/44704 (2013.01); G01N 2035/00158 (2013.01)] | 21 Claims |
1. A microfluidic device, comprising:
a reaction chamber;
a microfluidic channel in fluidic communication with the reaction chamber; and
a vial comprising:
a compartment extending between a first end and a second end opposite the first end, wherein the first end is above the second end;
an exit port at the second end of the compartment and in fluidic communication with the microfluidic channel;
a first movable stopper sealing the compartment from fluidic communication with an external environment through the first end; and
a second movable stopper sealing the compartment from fluidic communication with the microfluidic channel through the exit port;
wherein, in a state of the first movable stopper sealing the compartment and the second movable stopper sealing the compartment, the compartment is configured to hold a fluid composition between the first movable stopper and the second movable stopper; and
wherein the first movable stopper and the second movable stopper are configured to move relative to each other such that, in response to force applied to the first movable stopper to move the first movable stopper toward the second moveable stopper, the second moveable stopper is actuatable to unseal and place the compartment in fluidic communication with the microfluidic channel through the exit port.
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