US 12,168,210 B2
Devices, systems, and methods of electronic modulation of polymerase for DNA synthesis
Hua Wang, Atlanta, GA (US); and Andrew Hessel, Atlanta, GA (US)
Assigned to Georgia Tech Research Corporation, Atlanta, GA (US)
Appl. No. 17/055,803
Filed by Georgia Tech Research Corporation, Atlanta, GA (US)
PCT Filed May 17, 2019, PCT No. PCT/US2019/032844
§ 371(c)(1), (2) Date Nov. 16, 2020,
PCT Pub. No. WO2019/222612, PCT Pub. Date Nov. 21, 2019.
Claims priority of provisional application 62/672,660, filed on May 17, 2018.
Prior Publication US 2021/0229059 A1, Jul. 29, 2021
Int. Cl. B01J 19/00 (2006.01); C40B 50/14 (2006.01)
CPC B01J 19/0046 (2013.01) [C40B 50/14 (2013.01); B01J 2219/00596 (2013.01); B01J 2219/00626 (2013.01); B01J 2219/00653 (2013.01); B01J 2219/00659 (2013.01); B01J 2219/00673 (2013.01); B01J 2219/00689 (2013.01); B01J 2219/00713 (2013.01)] 12 Claims
 
1. A method of de novo nucleotide chain assembly comprising:
immobilizing a template-independent enzyme to an electrode platform comprising an electrode with a seeded inert surface functionalized with one or more links, the template-independent enzyme immobilized to the electrode platform via one or more of the links;
controlling, via direct electrical-field modulation, the immobilized template-independent enzyme to assemble a first nucleotide of a nucleotide chain by electrically modulating the electrode; and
controlling, via direct electrical-field modulation, the immobilized template-independent enzyme to assemble a next nucleotide of the nucleotide chain by electrically modulating the electrode;
wherein the assembly of the nucleotide chain is a de novo assembly of the nucleotide chain without using primer and without using template enzymes.