US 12,168,791 B2
Method for modifying the glycosylation profile of a recombinant glycoprotein produced in cell culture
László Párta, Saint-Louis (FR); Ákos Putics, Budapest (HU); Tibor Balogh, Budapest (HU); Balázs Horváth, Ajka (HU); and Gáspár Nagy, Gyömr (HU)
Assigned to RICHTER GEDEON NYRT., Budapest (HU)
Appl. No. 17/292,302
Filed by RICHTER GEDEON NYRT., Budapest (HU)
PCT Filed Nov. 6, 2019, PCT No. PCT/EP2019/080342
§ 371(c)(1), (2) Date May 7, 2021,
PCT Pub. No. WO2020/094694, PCT Pub. Date May 14, 2020.
Claims priority of application No. 1800376 (HU), filed on Nov. 7, 2018.
Prior Publication US 2022/0098634 A1, Mar. 31, 2022
Int. Cl. C12P 21/00 (2006.01); C12N 5/00 (2006.01)
CPC C12P 21/005 (2013.01) [C12N 5/0043 (2013.01); C12N 2500/20 (2013.01); C12N 2500/33 (2013.01); C12N 2500/34 (2013.01); C12N 2511/00 (2013.01)] 21 Claims
 
1. A method for modifying the glycosylation profile of a recombinant glycoprotein produced in cell culture comprising:
culturing eukaryotic cells expressing the recombinant glycoprotein in a cell culture medium, wherein the cell culture medium is supplemented with fucose, manganese, and taurine,
wherein the glycosylation profile comprises a fucosylation profile and/or galactosylation profile, and
wherein the fucosylation profile and/or galactosylation profile of the produced recombinant glycoprotein is modified to better resemble the fucosylation profile and/or galactosylation profile_of a reference glycoprotein than when the eukaryotic cells expressing the recombinant glycoprotein are cultured without fucose, manganese, and taurine supplementation.