US 12,168,679 B2
Modified bacteria for enhanced bioproduction and methods of using the same
William H. Bothfeld, Chicago, IL (US); and Keith E. J. Tyo, Evanston, IL (US)
Assigned to Northwestern University, Evanston, IL (US)
Filed by Northwestern University, Evanston, IL (US)
Filed on Nov. 9, 2021, as Appl. No. 17/522,441.
Claims priority of provisional application 63/111,851, filed on Nov. 10, 2020.
Prior Publication US 2022/0144899 A1, May 12, 2022
Int. Cl. C07K 14/245 (2006.01); C12N 15/70 (2006.01); C12P 5/00 (2006.01); C12P 7/42 (2006.01)
CPC C07K 14/245 (2013.01) [C12N 15/70 (2013.01); C12P 5/002 (2013.01); C12P 7/42 (2013.01)] 25 Claims
 
1. A modified bacterium comprising a modification to the expression of at least ten genes encoding a type I or type II toxin-antitoxin (TA) system protein (Δ10TA) wherein the modification reduces the activity of a toxin protein or suppresses the effects of a toxin protein, and at least two genes encoding structural proteins that modulate ribosomes selected from rmf (Δrmf), hpf (Δhpf), rsfs (Δrsfs), yqjD (ΔyqjD), elaB (ΔelaB), raiA (ΔraiA), ygaM (ΔygaM), and SRA (ΔSRA), wherein the bacterium is Escherichia coli (E. coli).