CPC C12N 15/8271 (2013.01) [C12N 9/88 (2013.01); C12N 15/8243 (2013.01); C12N 15/8262 (2013.01); C12Y 401/01031 (2013.01); C12N 2820/002 (2013.01); C12N 2820/007 (2013.01)] | 6 Claims |
1. A method for producing a maize plant expressing a variant phosphoenolpyruvate carboxylase (PEPC), comprising:
(a) introducing a clustered regularly interspaced short palindromic repeats (CRISPR) associated protein (Cas9) genome-editing system into a maize plant cell to form a transformed maize plant cell comprising a nucleic acid encoding the variant PEPC comprising (i) an amino acid substitution selected from the group consisting of A651V, G678S, and T778I in the consensus sequence of SEQ ID NO:8, and/or (ii) an amino acid substitution selected from the group consisting of A776S and R886G in the consensus sequence of SEQ ID NO:8; and
(b) regenerating a maize plant from the transformed plant cell,
wherein the maize plant expresses the variant PEPC and has enhanced aluminum resistance as compared to a control maize plant that does not express the variant PEPC.
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