CPC G01N 33/57488 (2013.01) | 4 Claims |
1. A method of identifying 2-hydroxygluterate (2-hg) metabolites in a sample comprising:
a) obtaining the sample from a patient, wherein the biological fluid is selected from at least one of: serum, urine, cerebrospinal fluid, or sputum;
b) extracting the 2-hydroxygluterate (2-hg) metabolites from the sample;
c) derivatizing the 2-hydroxygluterate (2-hg) metabolites with trimethylsilyldiazomethane (TMSDAM) and methanol;
d) separating the one or more 2-hg metabolites in the sample using a chiral gas chromatography capillary column; and
e) quantitating an amount of 2-hg metabolites and enantiomers in the sample using mass spectrometry (MS).
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