CPC C12N 1/125 (2021.05) [C12R 2001/89 (2021.05)] | 8 Claims |
1. A process for production of enriched algal biomass having high lipid productivity, the process consisting of:
i. providing an active starter culture of a microalgal strain;
ii. inoculating 10% v/v of the microalgal strain of step (i) in a nutrient medium in a fermentor with a substrate, wherein the nutrient medium consists of potassium ions in a range of 5-150 mM, magnesium ions in a range of 4-10 mM, copper ions in a range of 0.4-0.9 mM, calcium ions in a range of 0.3-0.9 mM, sulphate ions in a range of 5.6-15 mM, urea in a range of 14-40 mM, trace elements selected from the group consisting of FeCl3, CoCl2, H3BO3, Na2MoO4, ZnCl2, and Na2WO4, and vitamins selected from the group consisting of biotin, folic acid, pyridoxine HCl, thiamine HCl, riboflavin, nicotinic acid, B12, and thioctic acid;
iii. fermenting the nutrient medium of step (ii) at a temperature ranging from 20-37° C. at an aeration of 0.75-1 v/v/min and regulating stirring in a range from 150 to 900 rpm to keep dissolved oxygen at 20% throughout a continuous run;
iv. maintaining a pH value of the nutrient medium of step (iii) in a range of 6.5-9.5 and a concentration of a residual substrate in a range of 4-15 g/L inside the fermentor;
v. allowing the active starter culture to grow in a logarithmic phase in a steady state continuous run; and
vi. obtaining algal biomass enriched with omega-3 containing lipids from the fermentor.
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