CPC C12Q 1/6806 (2013.01) [C12N 15/1006 (2013.01); C12Q 1/689 (2013.01)] | 20 Claims |
1. A method of processing a biological sample, the method comprising the steps of:
(a) mixing the biological sample with an alkaline composition that lyses cells and denatures DNA to create a first liquid composition,
wherein the first liquid composition has a pH in the range of from pH 12.0 to pH 13.5, and
wherein the first liquid composition is free of detergents;
(b) mixing the first liquid composition with a pH buffered detergent reagent to create a second liquid composition having a pH lower than pH 9.5,
wherein the pH buffered detergent reagent comprises a pH buffer, a detergent, and a solid support particle that captures DNA; and
(c) isolating the solid support particle, and any DNA captured thereon, from the second liquid composition.
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