US 12,168,786 B2
Inhibition of DNA polymerases by uracil-DNA glycosylase-cleavable oligonucleotide ligands
Igor V. Kutyavin, Woodinville, WA (US); and Sergey G. Lokhov, Bothell, WA (US)
Assigned to Cepheid, Sunnyvale, CA (US)
Filed by Cepheid, Sunnyvale, CA (US)
Filed on Mar. 7, 2022, as Appl. No. 17/688,276.
Application 17/688,276 is a division of application No. 16/914,176, filed on Jun. 26, 2020, granted, now 11,268,075.
Application 16/914,176 is a division of application No. 16/210,643, filed on Dec. 5, 2018, granted, now 10,724,017, issued on Jul. 28, 2020.
Claims priority of provisional application 62/595,547, filed on Dec. 6, 2017.
Prior Publication US 2022/0251524 A1, Aug. 11, 2022
Int. Cl. C07H 21/02 (2006.01); C12N 9/12 (2006.01); C12N 9/24 (2006.01); C12N 9/99 (2006.01); C12N 15/113 (2010.01); C12N 15/115 (2010.01); C12Q 1/686 (2018.01)
CPC C12N 9/1252 (2013.01) [C12N 9/2497 (2013.01); C12N 9/99 (2013.01); C12N 15/113 (2013.01); C12N 15/115 (2013.01); C12Q 1/686 (2013.01); C12N 2310/16 (2013.01); C12N 2310/531 (2013.01); C12Y 207/07007 (2013.01)] 12 Claims
 
1. An oligonucleotide aptamer, comprising a DNA polymerase-binding nucleic acid sequence that is capable of forming a hairpin structure having a stem sequence portion and a loop sequence portion, wherein the loop sequence portion comprises a nucleotide sequence 5′-TTCTTAGCGTTT-3′ (SEQ ID NO:23) wherein one or more thymidine nucleotides at positions 1, 2, 10, 11 and 12 of the SEQ ID NO:23 sequence are substituted by one or more deoxyuridine nucleotides modifiable by a uracil-DNA glycosylase enzymatic activity.