CPC B01J 19/0046 (2013.01) [C12N 9/1264 (2013.01); C12P 19/34 (2013.01); C12Q 1/6806 (2013.01); C12Q 1/6811 (2013.01); C12Q 1/6837 (2013.01); B01J 2219/00596 (2013.01); B01J 2219/00722 (2013.01); C12Q 2525/101 (2013.01); C12Q 2525/117 (2013.01); C12Q 2525/186 (2013.01); C12Y 207/07019 (2013.01); C12Y 207/07031 (2013.01)] | 13 Claims |
1. A method for synthesizing oligonucleotides on a reusable solid support, the method comprising:
exposing a nucleic acid initiator attached to a solid support to nucleotide analogs in the presence of a polymerase to create a first oligonucleotide, wherein the nucleic acid initiator comprises a sequence-specific cleavage element;
contacting the nucleic acid initiator with a releasing agent to cleave the sequence-specific cleavage element and release the first oligonucleotide from the nucleic acid initiator;
dephosphorylating a 3′-end of the solid support-attached nucleic acid initiator; and
enzymatically renewing the sequence-specific cleavage element.
|