US 12,168,772 B2
Method of transforming photosynthetic organisms
John H. Verruto, San Diego, CA (US); and Jessica N. Weir, San Diego, CA (US)
Assigned to Viridos, Inc., La Jolla, CA (US)
Filed by VIRIDOS, INC., La Jolla, CA (US)
Filed on May 4, 2021, as Appl. No. 17/307,824.
Claims priority of provisional application 63/020,414, filed on May 5, 2020.
Prior Publication US 2021/0348183 A1, Nov. 11, 2021
Int. Cl. C12N 15/82 (2006.01); C12N 1/12 (2006.01); C12N 9/10 (2006.01); C12Q 1/6806 (2018.01); C12R 1/89 (2006.01)
CPC C12N 15/8269 (2013.01) [C12N 1/125 (2021.05); C12N 9/1007 (2013.01); C12Q 1/6806 (2013.01); C12R 2001/89 (2021.05); C12Y 201/01072 (2013.01)] 14 Claims
 
1. A method of transforming a photosynthetic Trebouxiophyte organism comprising:
contacting at least one DNA fragment with a dam methyltransferase to produce at least one methylated DNA fragment; and
transforming the photosynthetic Trebouxiophyte organism with the at least one methylated DNA fragment.