US 12,168,042 B2
Circular strained zymogen compositions and methods of use
Ronald Raines, Cambridge, MA (US); and Ian Windsor, Brighton, MA (US)
Assigned to Massachusetts Institute of Technology, Cambridge, MA (US); and Wisconsin Alumni Research Foundation, Madison, WI (US)
Filed by Massachusetts Institute of Technology, Cambridge, MA (US); and Wisconsin Alumni Research Foundation, Madison, WI (US)
Filed on Jun. 26, 2020, as Appl. No. 16/913,924.
Claims priority of provisional application 62/868,841, filed on Jun. 28, 2019.
Prior Publication US 2020/0405829 A1, Dec. 31, 2020
Int. Cl. C12N 9/22 (2006.01); A61K 38/55 (2006.01)
CPC A61K 38/55 (2013.01) [C12N 9/22 (2013.01)] 14 Claims
 
1. A circular zymogen of ribonuclease 1 (RNase 1) having a truncated C-terminus and a truncated N-terminus that are connected by a bridge that comprises a proteolytic cleavage site,
wherein the truncated C-terminus has at least 4 residues truncated and the truncated N-terminus has 1 to 6 residues truncated.