US 12,169,197 B2
Thread spray ambient ionization
Abraham Badu-Tawiah, Columbus, OH (US); Devin Swiner, Upper Marlboro, MD (US); and Sierra Jackson, New Orleans, LA (US)
Assigned to Ohio State Innovation Foundation, Columbus, OH (US)
Appl. No. 17/600,834
Filed by Ohio State Innovation Foundation, Columbus, OH (US)
PCT Filed Apr. 1, 2020, PCT No. PCT/US2020/026131
§ 371(c)(1), (2) Date Oct. 1, 2021,
PCT Pub. No. WO2020/205945, PCT Pub. Date Oct. 8, 2020.
Claims priority of provisional application 62/827,395, filed on Apr. 1, 2019.
Prior Publication US 2022/0187284 A1, Jun. 16, 2022
Int. Cl. G01N 33/53 (2006.01); G01N 1/10 (2006.01); G01N 1/40 (2006.01); G01N 33/68 (2006.01); H01J 49/02 (2006.01); H01J 49/04 (2006.01); H01J 49/16 (2006.01)
CPC G01N 33/53 (2013.01) [G01N 1/10 (2013.01); G01N 1/4044 (2013.01); G01N 33/6848 (2013.01); H01J 49/022 (2013.01); H01J 49/0409 (2013.01); H01J 49/165 (2013.01)] 18 Claims
OG exemplary drawing
 
1. A method for ionizing a compound, comprising:
(a) contacting a thread with a sample comprising an antigen, wherein the thread is functionalized with an antibody sufficient to capture the antigen;
(b) forming an antibody-antigen complex and contacting the antibody-antigen complex with a reporter antibody to form a sandwich complex;
(c) disposing the thread in a non-conductive chamber; and
(d) applying a direct current voltage to the thread.