US 12,169,204 B2
Coupled native mass spectrometry for antibody analysis
Yuetian Yan, Chappaqua, NY (US); and Shunhai Wang, Scarsdale, NY (US)
Assigned to Regeneron Pharmaceuticals, Inc., Tarrytown, NY (US)
Filed by Regeneron Pharmaceuticals, Inc., Tarrytown, NY (US)
Filed on Dec. 4, 2023, as Appl. No. 18/527,919.
Application 18/527,919 is a continuation of application No. 17/981,839, filed on Nov. 7, 2022, granted, now 11,885,811.
Application 17/981,839 is a continuation of application No. 17/033,820, filed on Sep. 27, 2020, granted, now 11,525,833, issued on Dec. 13, 2022.
Claims priority of provisional application 62/907,465, filed on Sep. 27, 2019.
Prior Publication US 2024/0110924 A1, Apr. 4, 2024
Int. Cl. G01N 30/72 (2006.01); G01N 33/68 (2006.01); H01J 49/00 (2006.01)
CPC G01N 33/6848 (2013.01) [G01N 30/72 (2013.01); H01J 49/0036 (2013.01)] 15 Claims
OG exemplary drawing
 
1. A method for determining oxidation profiles for at least one peptide or protein in a sample, comprising:
contacting the sample to a solid surface, wherein the solid surface comprises a hydrophobic group and the solid surface is included in a chromatography column;
washing the solid surface using a mobile phase to produce at least one eluent, wherein the eluent comprises the at least one peptide or protein;
determining the oxidation profile of the at least one peptide or protein using a mass spectrometer; and
determining the oxidation variants in the sample;
wherein the mass spectrometer is coupled online to the chromatography column.