CPC A61F 2/30 (2013.01) [B33Y 70/10 (2020.01); B33Y 80/00 (2014.12); C08L 89/00 (2013.01); A61F 2002/30062 (2013.01); A61F 2310/00371 (2013.01); B33Y 10/00 (2014.12); B82Y 5/00 (2013.01); B82Y 40/00 (2013.01)] | 17 Claims |
1. A method to fabricate chemically uncrosslinked collagen microparticles, wherein the method comprises:
a) combining an oil and either sorbitan monooleate or sorbitan monostearate and stirring to create a printing solution;
b) adding a collagen bioink composition dropwise to the printing solution to create a combined solution, wherein the collagen bioink composition comprises collagen, a polar solvent, and a stabilizing divalent ion chelating agent, wherein the collagen bioink fibrilizes when introduced into the printing solution;
c) stirring the combined solution;
d) increasing the temperature of the combined solution to 37° C. while continuing stirring; and
e) centrifuging the combined solution to collect the microparticles, wherein the combined solution is free of crosslinker or up to 0.05 mM of crosslinker is present.
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