US 12,167,965 B2
Liquid collagen bioinks and methods to make and use collagen structures
Sheila Grant, Columbia, MO (US); Colten Snider, Columbia, MO (US); and David Grant, Columbia, MO (US)
Assigned to The Curators of the University of Missouri, Columbia, MO (US)
Filed by The Curators of the University of Missouri, Columbia, MO (US)
Filed on Dec. 7, 2021, as Appl. No. 17/643,046.
Claims priority of provisional application 63/122,149, filed on Dec. 7, 2020.
Prior Publication US 2022/0175534 A1, Jun. 9, 2022
Int. Cl. A61F 2/30 (2006.01); B33Y 10/00 (2015.01); B33Y 70/10 (2020.01); B33Y 80/00 (2015.01); B82Y 5/00 (2011.01); B82Y 40/00 (2011.01); C08L 89/00 (2006.01)
CPC A61F 2/30 (2013.01) [B33Y 70/10 (2020.01); B33Y 80/00 (2014.12); C08L 89/00 (2013.01); A61F 2002/30062 (2013.01); A61F 2310/00371 (2013.01); B33Y 10/00 (2014.12); B82Y 5/00 (2013.01); B82Y 40/00 (2013.01)] 17 Claims
OG exemplary drawing
 
1. A method to fabricate chemically uncrosslinked collagen microparticles, wherein the method comprises:
a) combining an oil and either sorbitan monooleate or sorbitan monostearate and stirring to create a printing solution;
b) adding a collagen bioink composition dropwise to the printing solution to create a combined solution, wherein the collagen bioink composition comprises collagen, a polar solvent, and a stabilizing divalent ion chelating agent, wherein the collagen bioink fibrilizes when introduced into the printing solution;
c) stirring the combined solution;
d) increasing the temperature of the combined solution to 37° C. while continuing stirring; and
e) centrifuging the combined solution to collect the microparticles, wherein the combined solution is free of crosslinker or up to 0.05 mM of crosslinker is present.