CPC C12Q 1/6825 (2013.01) [C12Q 1/68 (2013.01); C12Q 1/6851 (2013.01); G01N 21/6486 (2013.01); G16B 5/00 (2019.02); G16B 25/00 (2019.02); G16B 25/20 (2019.02); G16B 40/10 (2019.02); G06F 17/10 (2013.01); G06F 17/11 (2013.01); G16B 40/00 (2019.02); Y02A 50/30 (2018.01)] | 30 Claims |
1. A system comprising:
a sample chamber configured to house a sample and analyte-specific reagent mixtures of analyte-specific hybridization probes and multiple fluorophores;
a multi-channel detector to detect:
a first electromagnetic signal at a first wavelength from the sample chamber, the first electromagnetic signal generated by excitement of a first fluorophore of the multiple fluorophores;
a second electromagnetic signal at a second wavelength from the sample chamber, the second electromagnetic signal generated by excitement of a second fluorophore of the multiple fluorophores;
a third electromagnetic signal at a third wavelength from the sample chamber, the third electromagnetic signal generated by excitement of a third fluorophore of the multiple fluorophores;
a fourth electromagnetic signal at a fourth wavelength from the sample chamber, the fourth electromagnetic signal generated by excitement of a fourth fluorophore of the multiple fluorophores;
a processor controlled analyzer to receive, from the multi-channel detector, a cumulative signal based on the first, second, third, and fourth electromagnetic signals and apply a decoding matrix to the cumulative signal to unambiguously detect the presence or absence of at least each of M analytes by associating, for each analyte, a first value in a first component of the cumulative signal and a second value in a second component of the cumulative signal, wherein each first value is an intensity or range of intensities and each second value is a wavelength or a range of wavelengths, and wherein the second values comprise the first, second, third, and fourth wavelengths, and the determination is made without immobilization, mass spectrometry or melting curve analysis;
wherein for the positive integer M,
M=C*log2 (F+1),
F is a positive integer and is equal to the maximum cumulative intensity of the first component of the signal, for any second value, when all of the analytes are present, and
C=4, 5, or 6; and
wherein F+1 is a positive integer and wherein F+1 is a power of 2,
wherein M is greater than the number of the second values used to encode the analytes (C), the multi-channel detector comprises C channels, and M and C are positive integers.
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