US 12,168,030 B2
Method for producing stable Actinidia deliciosa powder with high level bioactive actinidin enzyme
Craig Lachlan McIntosh, Christchurch (NZ); and Raumiria Pohatu, Christchurch (NZ)
Assigned to WAITAKI BIOSCIENCES, Christchurch (NZ)
Filed by Waitaki Biosciences, Christchurch (NZ)
Filed on Feb. 3, 2021, as Appl. No. 17/166,515.
Claims priority of provisional application 62/969,952, filed on Feb. 4, 2020.
Prior Publication US 2021/0322500 A1, Oct. 21, 2021
Int. Cl. A23L 19/00 (2016.01); A61K 36/185 (2006.01); A61K 38/48 (2006.01)
CPC A61K 36/185 (2013.01) [A61K 38/4873 (2013.01); A61K 2236/15 (2013.01); A61K 2236/17 (2013.01); A61K 2236/51 (2013.01)] 11 Claims
OG exemplary drawing
 
1. A method for producing stable and bioavailable Actinidia deliciosa powder from kiwifruit, comprising the steps of:
a) forming a minced kiwifruit pulp by mincing the kiwifruit pulp at a temperature of less than −18° C.;
b) freeze-drying the minced kiwifruit pulp by
i) a freezing step comprising blast freezing the minced kiwifruit pulp to a temperature of less than −30° C. in a period of less than 3 hours; and
ii) a drying step comprising drying the minced kiwifruit pulp to form dried pulp in
a first stage at 30° C. for 60 minutes, a second stage at 50° C. for 250 minutes, a third stage at 60° C. for 580 minutes, a fourth stage at 50° C. for 1320 minutes, a fifth stage at 40° C. for 640 minutes, and a sixth stage at 30° C. for 30 minutes; or
a first stage at 30° C. for 55 minutes, a second stage at 40° C. for 80 minutes, a third stage at 50° C. for 2260 minutes, a fourth stage at 40° C. for 455 minutes, and a fifth stage at 30° C. for 30 minutes;
wherein the dried pulp has an enzyme activity of at least 150,000 AU/g;
c) separately freeze-drying kiwifruit skin to form a dried skin that has a water activity of less than 0.2 or a moisture level of less than 1%;
d) measuring insoluble fiber content of the dried pulp and the dried skin; and
e) forming a blend by blending the dried skin and dried pulp by adding the dried skin to the dried pulp in an amount to obtain an insoluble fiber content of 10-15% in the blend.