CPC C07K 16/283 (2013.01) [C07K 16/08 (2013.01); C07K 16/12 (2013.01); C07K 16/30 (2013.01); C12N 15/63 (2013.01); C40B 40/10 (2013.01); A61P 35/00 (2018.01); C07K 2317/524 (2013.01); C07K 2317/526 (2013.01); C07K 2317/565 (2013.01); C07K 2319/55 (2013.01)] | 13 Claims |
1. An IgG1 Fc monomer polypeptide comprising CH2 and CH3 domains, wherein the polypeptide comprises arginine (R), histidine (H), lysine (K) and threonine (T) at positions 366, 368, 395 and 409 of IgG1, respectively, wherein the numbering is according to EU numbering and the Fc monomer polypeptide is capable of binding to a neonatal Fc receptor (FcRn), wherein the binding affinity of the IgG1 Fc monomer polypeptide to at least one antigen selected from the group consisting of 5T4, PD-L1 and OX40 is lower than that of a wild-type IgG1 Fc, as measured by an enzyme-linked immunosorbent assay (ELISA) with 100 ng of the antigen and up to 223 nM of the IgG1 Fc monomer polypeptide.
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