US 12,168,795 B2
Method for measuring cholesterol in low-density lipoprotein, measurement reagent, and measurement kit
Mizuki Miura, Shizuoka (JP); Tomoko Aratake, Shizuoka (JP); and Kenta Kinjo, Shizuoka (JP)
Assigned to HITACHI CHEMICAL DIAGNOSTICS SYSTEMS CO., LTD., Tokyo (JP)
Appl. No. 16/640,231
Filed by Hitachi Chemical Diagnostics Systems Co., Ltd., Tokyo (JP)
PCT Filed Aug. 30, 2018, PCT No. PCT/JP2018/032117
§ 371(c)(1), (2) Date Feb. 19, 2020,
PCT Pub. No. WO2019/044973, PCT Pub. Date Mar. 7, 2019.
Claims priority of application No. 2017-168235 (JP), filed on Sep. 1, 2017.
Prior Publication US 2020/0392558 A1, Dec. 17, 2020
Int. Cl. C12Q 1/60 (2006.01); C12Q 1/32 (2006.01); C12Q 1/44 (2006.01)
CPC C12Q 1/60 (2013.01) [C12Q 1/32 (2013.01); C12Q 1/44 (2013.01); G01N 2333/902 (2013.01); G01N 2333/918 (2013.01); G01N 2405/00 (2013.01)] 18 Claims
 
1. A method for measuring cholesterol in low-density lipoprotein in a sample, the method comprising:
mixing the sample and an aqueous solvent which comprises:
(i) a combination of cholesterol ester hydrolase and cholesterol oxidase or (ii) a combination of cholesterol ester hydrolase, an oxidized coenzyme and cholesterol dehydrogenase,
one or more surfactants selected from the group consisting of a polyoxyethylene alkyl ether and a polyoxyethylene polyoxypropylene alkyl ether,
a polyoxyethylene polyoxypropylene copolymer and
a polyoxypropylene derivative not comprising any polyoxyethylene in a molecule, to react cholesterol in low-density lipoprotein in the sample with (i) the combination of cholesterol ester hydrolase and cholesterol oxidase or (ii) the combination of cholesterol ester hydrolase, an oxidized coenzyme and cholesterol dehydrogenase; and
measuring a substance formed or consumed in the reaction from the low-density lipoprotein,
with the proviso that the aqueous solvent does not comprise any surfactant having an alkylphenol structure.