US 12,168,787 B2
Process of extracting oil from thin stillage
Nathaniel Edward Kreel, Louisburg, NC (US); Joseph Mark Jump, Raleigh, NC (US); Bernardo Vidal, Jr., Wake Forest, NC (US); Chee-Leong Soong, Raleigh, NC (US); Madison Roberts, Raleigh, NC (US); Melissa Carrie Hooss, Franklinton, NC (US); and Xinyu Shen, Wake Forest, NC (US)
Assigned to Novozymes A/S, Bagsvaerd (DK)
Filed by Novozymes A/S, Bagsvaerd (DK)
Filed on May 8, 2023, as Appl. No. 18/313,955.
Application 18/313,955 is a continuation of application No. 17/306,001, filed on May 3, 2021, granted, now 11,674,127.
Application 17/306,001 is a continuation of application No. 16/787,203, filed on Feb. 11, 2020, granted, now 11,028,378, issued on Jun. 8, 2021.
Application 16/787,203 is a continuation of application No. 16/062,519, granted, now 10,597,645, issued on Mar. 24, 2020, previously published as PCT/US2016/067080, filed on Dec. 16, 2016.
Claims priority of provisional application 62/271,182, filed on Dec. 22, 2015.
Claims priority of provisional application 62/271,063, filed on Dec. 22, 2015.
Claims priority of provisional application 62/324,107, filed on Apr. 18, 2016.
Claims priority of provisional application 62/430,695, filed on Dec. 6, 2016.
Prior Publication US 2023/0340432 A1, Oct. 26, 2023
This patent is subject to a terminal disclaimer.
Int. Cl. C12N 9/14 (2006.01); C12N 9/16 (2006.01); C12N 9/20 (2006.01); C12N 9/26 (2006.01); C12N 9/28 (2006.01); C12N 9/34 (2006.01); C12N 9/42 (2006.01); C12N 9/44 (2006.01); C12N 9/50 (2006.01); C12P 7/06 (2006.01); C12P 7/64 (2022.01); C12P 19/14 (2006.01)
CPC C12N 9/14 (2013.01) [C12N 9/16 (2013.01); C12N 9/20 (2013.01); C12N 9/2414 (2013.01); C12N 9/2417 (2013.01); C12N 9/2428 (2013.01); C12N 9/2437 (2013.01); C12N 9/2457 (2013.01); C12N 9/50 (2013.01); C12P 7/06 (2013.01); C12P 19/14 (2013.01); C12Y 301/03008 (2013.01); C12Y 301/04 (2013.01); C12Y 301/04011 (2013.01); C12Y 302/01001 (2013.01); C12Y 302/01003 (2013.01); C12Y 302/01004 (2013.01); C12Y 302/01008 (2013.01); C12Y 302/01041 (2013.01); C12Y 304/21062 (2013.01); C12Y 304/23023 (2013.01); C12Y 304/24039 (2013.01); C12P 7/64 (2013.01); Y02E 50/10 (2013.01)] 12 Claims
 
1. A process of recovering oil, comprising
(a) converting a starch-containing material into dextrins with an alpha-amylase;
(b) saccharifying the dextrins using a carbohydrate source generating enzyme to form a sugar;
(c) fermenting the sugar in a fermentation medium into a fermentation product using a fermenting organism;
(d) recovering the fermentation product to form a whole stillage;
(e) separating the whole stillage into thin stillage and wet cake;
(e′) optionally concentrating the thin stillage into syrup;
(f) recovering oil from the thin stillage and/or optionally the syrup, wherein a protease and a phospholipase are present and/or added during steps (a) to (c);
wherein the protease present and/or added in steps (a) to (c) is a protease having the amino acid sequence set forth in SEQ ID NO: 9 or a protease having at least 85% identity to SEQ ID NO: 9 and having protease activity; and
wherein the phospholipase present and/or added in steps (a) to (c) is:
(i) the phospholipase shown in SEQ ID NO: 15 or one having a sequence identity thereto of at least 90% and having phospholipase activity;
(ii) the phospholipase shown in SEQ ID NO: 16 or one having a sequence identity thereto of at least 90% and having phospholipase activity; or
(iii) the phospholipase shown in SEQ ID NO: 17 or one having a sequence identity thereto of at least 90% and having phospholipase activity.