US 12,168,793 B2
Method of enzymatically preparing n-3 polyunsaturated fatty acid diacylglycerol
Jialing Gu, Wuxi (CN); Xiaosan Wang, Wuxi (CN); Houyue Li, Wuxi (CN); Zhimian Liu, Wuxi (CN); Weijia Bao, Wuxi (CN); Zixin Wang, Wuxi (CN); Yifan Wang, Wuxi (CN); Yijie Fu, Wuxi (CN); and Yujie Su, Wuxi (CN)
Assigned to Jiangnan University, Wuxi (CN)
Filed by Jiangnan University, Wuxi (CN)
Filed on May 15, 2023, as Appl. No. 18/317,144.
Claims priority of application No. 202310164194.8 (CN), filed on Feb. 24, 2023.
Prior Publication US 2024/0287557 A1, Aug. 29, 2024
Int. Cl. C12P 7/6472 (2022.01); C12N 9/20 (2006.01); C12R 1/72 (2006.01)
CPC C12P 7/6472 (2013.01) [C12N 9/20 (2013.01); C12R 2001/72 (2021.05)] 10 Claims
 
1. A method for enzymatically preparing a n-3 polyunsaturated fatty acid diacylglycerol, comprising:
adding an oil rich in n-3 polyunsaturated fatty acid and the content of n-3 polyunsaturated fatty acid is 30% to 70% and water to a reactor for a high-pressure homogenization;
adding a lipase for undergoing an enzymatic reaction;
removing the water and the lipase after the reaction; and
separating free fatty acids and obtaining a diacylglycerol oil;
wherein the high-pressure homogenization is carried out at a homogenization pressure of at least 10 MPa for a homogenization time of at least 1 minute.