US 12,168,062 B2
Guide RNA that targets a mutant human guanylate cyclase 2A allele
Lior Izhar, Tel Aviv (IL); David Baram, Nir Zvi (IL); Joe Georgeson, Rehevot (IL); Michal Golan-Mashiach, Ness-Ziona (IL); Asael Herman, Ness-Ziona (IL); and Rafi Emmanuel, Ramla (IL)
Assigned to EmendoBio Inc., Wilmington, DE (US)
Filed by EmendoBio Inc., Wilmington, DE (US)
Filed on Nov. 28, 2018, as Appl. No. 16/203,004.
Claims priority of provisional application 62/680,479, filed on Jun. 4, 2018.
Claims priority of provisional application 62/591,333, filed on Nov. 28, 2017.
Prior Publication US 2023/0173106 A1, Jun. 8, 2023
Int. Cl. C12N 15/11 (2006.01); A61K 48/00 (2006.01); A61P 27/00 (2006.01); C12N 9/22 (2006.01); C12N 15/113 (2010.01)
CPC A61K 48/0066 (2013.01) [A61P 27/00 (2018.01); C12N 9/22 (2013.01); C12N 15/11 (2013.01); C12N 15/113 (2013.01); C12N 15/1137 (2013.01); C12Y 301/00 (2013.01)] 6 Claims
 
1. An isolated guide RNA (gRNA) that targets a mutant human guanylate cyclase 2D (GUCY2D) allele, wherein the gRNA comprises a CRISPR RNA (crRNA) consisting of the nucleic acid sequence of SEQ ID NO: 237, 238, 241, 242, 247, 248, 293, 294, 307, 413, 414, 417, 418, or 3011.