US 12,168,046 B2
Mycobacterial antigen compositions and methods of use
Michael Keller, Laurel, MD (US); Catherine Bollard, Bethesda, MD (US); Patrick Hanley, Washington, DC (US); Conrad Russell Cruz, Bethesda, MD (US); Haili Lang, Derwood, MD (US); and Shabnum Patel, Washington, DC (US)
Assigned to Children's National Medical Center, Washington, DC (US)
Appl. No. 17/050,829
Filed by Children's National Medical Center, Washington, DC (US)
PCT Filed Apr. 26, 2019, PCT No. PCT/US2019/029505
§ 371(c)(1), (2) Date Oct. 26, 2020,
PCT Pub. No. WO2019/210282, PCT Pub. Date Oct. 31, 2019.
Claims priority of provisional application 62/663,239, filed on Apr. 26, 2018.
Prior Publication US 2021/0290747 A1, Sep. 23, 2021
Int. Cl. A61K 39/04 (2006.01); A61P 31/06 (2006.01); C12N 5/0783 (2010.01); A61K 39/00 (2006.01)
CPC A61K 39/04 (2013.01) [A61P 31/06 (2018.01); C12N 5/0636 (2013.01); A61K 2039/5158 (2013.01); A61K 2039/70 (2013.01); C12N 2501/2304 (2013.01); C12N 2501/2307 (2013.01); C12N 2501/2315 (2013.01); C12N 2501/2321 (2013.01); C12N 2501/24 (2013.01); C12N 2501/25 (2013.01)] 3 Claims
 
1. A method of expanding T cells ex vivo, the method comprising:
culturing one or a plurality of T-cells;
contacting the one or plurality of T-cells with:
a polypeptide comprising an amino acid sequence coding for an Ag85B antigen at least 70% identical to SEQ ID NO: 6 from a Mycobacterium species,
a polypeptide comprising an amino acid sequence coding for a PPE68 antigen at least 70% identical to SEQ ID NO: 7 from a Mycobacterium species,
a polypeptide comprising an amino acid sequence coding for an ESXA antigen at least 70% identical to SEQ ID NO: 8 from a Mycobacterium species,
a polypeptide comprising an amino acid sequence coding for an ESXB antigen at least 70% identical to SEQ ID NO: 9 from a Mycobacterium species, and
a polypeptide comprising an amino acid sequence coding for an ADK antigen at least 70% identical to SEQ ID NO: 10 from a Mycobacterium species.