US 12,168,796 B2
Sample preparation method and system
Matthias Jost, San Diego, CA (US); Barbara L. Eaton, San Diego, CA (US); and Ankur Shah, San Diego, CA (US)
Assigned to GEN-PROBE INCORPORATED, San Diego, CA (US)
Appl. No. 17/255,500
Filed by GEN-PROBE INCORPORATED, San Diego, CA (US)
PCT Filed Jun. 14, 2019, PCT No. PCT/US2019/037245
§ 371(c)(1), (2) Date Dec. 23, 2020,
PCT Pub. No. WO2020/005584, PCT Pub. Date Jan. 2, 2020.
Claims priority of provisional application 62/691,478, filed on Jun. 28, 2018.
Prior Publication US 2021/0269854 A1, Sep. 2, 2021
Int. Cl. C12Q 1/6806 (2018.01); C12N 15/10 (2006.01); C12Q 1/689 (2018.01)
CPC C12Q 1/6806 (2013.01) [C12N 15/1006 (2013.01); C12Q 1/689 (2013.01)] 20 Claims
 
1. A method of processing a biological sample, the method comprising the steps of:
(a) mixing the biological sample with an alkaline composition that lyses cells and denatures DNA to create a first liquid composition,
wherein the first liquid composition has a pH in the range of from pH 12.0 to pH 13.5, and
wherein the first liquid composition is free of detergents;
(b) mixing the first liquid composition with a pH buffered detergent reagent to create a second liquid composition having a pH lower than pH 9.5,
wherein the pH buffered detergent reagent comprises a pH buffer, a detergent, and a solid support particle that captures DNA; and
(c) isolating the solid support particle, and any DNA captured thereon, from the second liquid composition.