US 12,168,231 B2
Method of analysis
Billy W. Colston, Jr., San Ramon, CA (US); Benjamin J. Hindson, Livermore, CA (US); Kevin D. Ness, Pleasanton, CA (US); and Donald A. Masquelier, Tracy, CA (US)
Assigned to Bio-Rad Laboratories, Inc., Hercules, CA (US)
Filed by Bio-Rad Laboratories, Inc., Hercules, CA (US)
Filed on Sep. 27, 2021, as Appl. No. 17/486,804.
Application 17/486,804 is a continuation of application No. 16/404,659, filed on May 6, 2019, granted, now 11,130,134.
Application 16/404,659 is a continuation of application No. 15/365,894, filed on Nov. 30, 2016, granted, now 10,279,350, issued on May 7, 2019.
Application 15/365,894 is a continuation of application No. 12/962,511, filed on Dec. 7, 2010, granted, now 9,623,384, issued on Apr. 18, 2017.
Application 12/962,511 is a continuation of application No. 12/586,626, filed on Sep. 23, 2009, granted, now 9,156,010, issued on Oct. 13, 2015.
Claims priority of provisional application 61/277,270, filed on Sep. 22, 2009.
Claims priority of provisional application 61/277,204, filed on Sep. 21, 2009.
Claims priority of provisional application 61/277,203, filed on Sep. 21, 2009.
Claims priority of provisional application 61/277,216, filed on Sep. 21, 2009.
Claims priority of provisional application 61/277,249, filed on Sep. 21, 2009.
Claims priority of provisional application 61/277,200, filed on Sep. 21, 2009.
Claims priority of provisional application 61/275,731, filed on Sep. 1, 2009.
Claims priority of provisional application 61/271,538, filed on Jul. 21, 2009.
Claims priority of provisional application 61/206,975, filed on Feb. 5, 2009.
Claims priority of provisional application 61/194,043, filed on Sep. 23, 2008.
Prior Publication US 2022/0008928 A1, Jan. 13, 2022
Int. Cl. B01L 3/00 (2006.01); B01F 23/41 (2022.01); B01F 33/3011 (2022.01); B01F 35/10 (2022.01); B01L 3/02 (2006.01); B01L 7/00 (2006.01); B29C 45/00 (2006.01); C12Q 1/686 (2018.01); G01N 21/3563 (2014.01); G01N 21/49 (2006.01); G01N 21/64 (2006.01); B01F 101/23 (2022.01); B29L 31/00 (2006.01)
CPC B01L 3/502784 (2013.01) [B01F 23/41 (2022.01); B01F 33/3011 (2022.01); B01F 35/10 (2022.01); B01L 3/0241 (2013.01); B01L 3/502715 (2013.01); B01L 7/525 (2013.01); B29C 45/0053 (2013.01); B29C 45/006 (2013.01); C12Q 1/686 (2013.01); G01N 21/3563 (2013.01); G01N 21/49 (2013.01); G01N 21/6428 (2013.01); G01N 21/6486 (2013.01); B01F 23/4143 (2022.01); B01F 23/4145 (2022.01); B01F 2101/23 (2022.01); B01L 7/52 (2013.01); B01L 2200/0673 (2013.01); B01L 2200/0689 (2013.01); B01L 2200/10 (2013.01); B01L 2200/12 (2013.01); B01L 2300/041 (2013.01); B01L 2300/0654 (2013.01); B01L 2300/0816 (2013.01); B01L 2300/0819 (2013.01); B01L 2300/0858 (2013.01); B01L 2300/0867 (2013.01); B01L 2300/1822 (2013.01); B01L 2400/0478 (2013.01); B01L 2400/0487 (2013.01); B01L 2400/049 (2013.01); B01L 2400/0622 (2013.01); B29C 2045/0079 (2013.01); B29L 2031/752 (2013.01); G01N 2021/6439 (2013.01); Y02A 90/10 (2018.01)] 16 Claims
OG exemplary drawing
 
1. A method of analysis, the method comprising:
selecting a microfluidic device defining a flow path extending from an inlet to an outlet;
introducing a sample-containing fluid into the flow path from a sample port via the inlet;
isolating volumes of the sample-containing fluid from one another on the flow path; and
imaging a two-dimensional monolayer of the volumes, the two-dimensional monolayer being formed along the flow path between the inlet and the outlet, wherein the flow path includes a chamber having an upper surface and a lower surface, and a height, a width, and an area, wherein the height corresponds in size to the volumes such that the two-dimensional monolayer of the volumes is formed in the chamber when the sample-containing fluid flows into the chamber, wherein the width is at least about ten times the height, and wherein the area is larger than the sample port.