CPC B01L 3/502784 (2013.01) [B01F 23/41 (2022.01); B01F 33/3011 (2022.01); B01F 35/10 (2022.01); B01L 3/0241 (2013.01); B01L 3/502715 (2013.01); B01L 7/525 (2013.01); B29C 45/0053 (2013.01); B29C 45/006 (2013.01); C12Q 1/686 (2013.01); G01N 21/3563 (2013.01); G01N 21/49 (2013.01); G01N 21/6428 (2013.01); G01N 21/6486 (2013.01); B01F 23/4143 (2022.01); B01F 23/4145 (2022.01); B01F 2101/23 (2022.01); B01L 7/52 (2013.01); B01L 2200/0673 (2013.01); B01L 2200/0689 (2013.01); B01L 2200/10 (2013.01); B01L 2200/12 (2013.01); B01L 2300/041 (2013.01); B01L 2300/0654 (2013.01); B01L 2300/0816 (2013.01); B01L 2300/0819 (2013.01); B01L 2300/0858 (2013.01); B01L 2300/0867 (2013.01); B01L 2300/1822 (2013.01); B01L 2400/0478 (2013.01); B01L 2400/0487 (2013.01); B01L 2400/049 (2013.01); B01L 2400/0622 (2013.01); B29C 2045/0079 (2013.01); B29L 2031/752 (2013.01); G01N 2021/6439 (2013.01); Y02A 90/10 (2018.01)] | 16 Claims |
1. A method of analysis, the method comprising:
selecting a microfluidic device defining a flow path extending from an inlet to an outlet;
introducing a sample-containing fluid into the flow path from a sample port via the inlet;
isolating volumes of the sample-containing fluid from one another on the flow path; and
imaging a two-dimensional monolayer of the volumes, the two-dimensional monolayer being formed along the flow path between the inlet and the outlet, wherein the flow path includes a chamber having an upper surface and a lower surface, and a height, a width, and an area, wherein the height corresponds in size to the volumes such that the two-dimensional monolayer of the volumes is formed in the chamber when the sample-containing fluid flows into the chamber, wherein the width is at least about ten times the height, and wherein the area is larger than the sample port.
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