CPC C12N 15/815 (2013.01) [C12N 15/905 (2013.01)] | 19 Claims |
1. A method of producing an antibody, the method comprising,
culturing a yeast host cell comprising (i) a nucleic acid sequence encoding an antibody in the yeast host cell genome and (ii) a circular extrachromosomal nucleic acid comprising a coding sequence for a selectable marker, under conditions suitable for production of the antibody, wherein the host cell further comprises a nucleic acid that encodes an RNA-guided DNA endonuclease, wherein the nucleic acid is integrated in a YKU70 gene, thereby reducing non-homologous end joining (NHEJ) activity in the host cell as compared to a host cell in which YKU70 is intact; and
recovering the antibody produced by the yeast host cell.
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