US 12,168,779 B2
Apparatus and method for cell kill confirmation
Frederick Savage, DeForest, WI (US); David Appleyard, DeForest, WI (US); Zheng Xia, DeForest, WI (US); Matthew D. Ebersole, DeForest, WI (US); and Daniel McAda, DeForest, WI (US)
Assigned to ABS Global, Inc., DeForest, WI (US)
Filed by ABS Global, Inc., DeForest, WI (US)
Filed on Jul. 14, 2023, as Appl. No. 18/352,593.
Application 18/352,593 is a continuation of application No. 17/812,369, filed on Jul. 13, 2022, granted, now 11,753,620.
Application 17/812,369 is a continuation of application No. 16/442,058, filed on Jun. 14, 2019, granted, now 11,427,804, issued on Aug. 30, 2022.
Claims priority of provisional application 62/685,614, filed on Jun. 15, 2018.
Prior Publication US 2023/0365924 A1, Nov. 16, 2023
Int. Cl. G01N 15/14 (2024.01); C12N 5/00 (2006.01); G01N 21/64 (2006.01); G02B 21/16 (2006.01)
CPC C12N 5/0093 (2013.01) [G01N 15/147 (2013.01); G01N 21/6456 (2013.01); G02B 21/16 (2013.01); G01N 2021/646 (2013.01)] 16 Claims
OG exemplary drawing
 
1. A method for producing a sexed semen sample, the method comprising:
illuminating a cell with a focused beam of electromagnetic radiation;
detecting a first fluorescence by the cell;
determining a set of characteristics of the cell based on the fluorescence of the cell;
identifying the cell as an undesired cell based on the set of characteristics;
setting a timing signal for a kill laser wherein the timing signal causes the kill laser to emit a focused beam of electromagnetic radiation at the cell;
firing the kill laser at the cell based on the timing signal;
detecting a second fluorescence by the cell;
determining a type of event based on the second fluorescence, wherein the type of event is one of a slice, a kill, or a miss.