US 12,169,198 B2
Systems and methods for sample analysis
Andrew D. Price, Hayward, CA (US); and Christopher Hindson, Livermore, CA (US)
Assigned to 10X GENOMICS, INC., Pleasanton, CA (US)
Filed by 10X GENOMICS, INC., Pleasanton, CA (US)
Filed on Aug. 18, 2023, as Appl. No. 18/235,616.
Application 18/235,616 is a continuation of application No. 16/737,762, filed on Jan. 8, 2020, abandoned.
Claims priority of provisional application 62/789,893, filed on Jan. 8, 2019.
Prior Publication US 2024/0044877 A1, Feb. 8, 2024
Int. Cl. G01N 33/532 (2006.01); C07K 14/74 (2006.01); C12N 15/10 (2006.01); C12N 15/11 (2006.01); C12N 15/85 (2006.01); C12Q 1/6804 (2018.01); C12Q 1/6806 (2018.01); C12Q 1/6818 (2018.01); C12Q 1/6827 (2018.01); C12Q 1/6881 (2018.01); C40B 50/06 (2006.01); G01N 33/50 (2006.01); G01N 33/53 (2006.01); G01N 33/543 (2006.01); G01N 33/548 (2006.01); G01N 33/569 (2006.01); G01N 33/58 (2006.01); C40B 30/04 (2006.01); C40B 70/00 (2006.01)
CPC G01N 33/532 (2013.01) [C07K 14/70539 (2013.01); C12N 15/1037 (2013.01); C12N 15/1055 (2013.01); C12N 15/1065 (2013.01); C12N 15/1075 (2013.01); C12N 15/11 (2013.01); C12N 15/85 (2013.01); C12Q 1/6804 (2013.01); C12Q 1/6806 (2013.01); C12Q 1/6818 (2013.01); C12Q 1/6827 (2013.01); C12Q 1/6881 (2013.01); G01N 33/5032 (2013.01); G01N 33/505 (2013.01); G01N 33/5304 (2013.01); G01N 33/5306 (2013.01); G01N 33/5308 (2013.01); G01N 33/54306 (2013.01); G01N 33/54366 (2013.01); G01N 33/548 (2013.01); G01N 33/56977 (2013.01); G01N 33/58 (2013.01); C12N 2310/20 (2017.05); C12N 2320/10 (2013.01); C12Q 2537/164 (2013.01); C12Q 2563/179 (2013.01); C12Q 2563/185 (2013.01); C12Q 2565/1015 (2013.01); C40B 30/04 (2013.01); C40B 50/06 (2013.01); C40B 70/00 (2013.01)] 22 Claims
 
1. A mixture, comprising:
a nucleic acid comprising: (i) a first moiety, (ii) a nucleic acid sequence, and (ii) a poly-thymine (poly(T)) sequence, a poly-guanine (poly(G)) sequence, or an N-mer sequence;
a nucleic acid barcode comprising a first strand and a second strand, wherein said first strand comprises a second moiety and a barcode sequence, wherein said second strand comprises (1) a first sequence complementary to said barcode sequence and (2) an overhang comprising a second sequence complementary to said nucleic acid sequence; and
reagents configured to non-enzymatically ligate said first moiety to said second moiety.