CPC C12Q 1/005 (2013.01) [C12Q 1/28 (2013.01); G01N 2333/4737 (2013.01)] | 18 Claims |
1. A method for detecting a binding event between an immobilised primary antibody and a target analyte-secondary antibody-enzyme complex using an electrochemical measurement technique, the method comprising:
incubating a target analyte, a secondary antibody and an enzyme label in a single solution;
contacting a primary antibody immobilised on an electrode surface with the single solution of the target analyte, the secondary antibody and the enzyme label to create a sandwich complex bound to the electrode surface;
contacting the sandwich complex with a substrate for the enzyme, wherein the substrate is converted by the enzyme into an insoluble precipitate on the electrode surface; and
measuring an electrochemical signal during and/or after the formation of the insoluble precipitate on the electrode surface.
|