US 12,168,775 B2
Compositions and methods comprising male fertility sequences
Marc C Albertsen, Grimes, IA (US); Andrew Mark Cigan, Madison, WI (US); Howard P Hershey, West Chester, PA (US); Michael Lassner, Portland, OR (US); and Yongzhong Wu, Johnston, IA (US)
Assigned to PIONEER HI-BRED INTERNATIONAL, INC., , IA (US)
Filed by PIONEER HI-BRED INTERNATIONAL, INC., Johnston, IA (US)
Filed on Jun. 2, 2022, as Appl. No. 17/805,226.
Application 17/805,226 is a continuation of application No. 16/189,499, filed on Nov. 13, 2018, granted, now 11,377,668.
Application 16/189,499 is a continuation of application No. 14/425,916, granted, now 10,155,962, issued on Dec. 18, 2018, previously published as PCT/US2013/058500, filed on Sep. 6, 2013.
Claims priority of provisional application 61/697,590, filed on Sep. 6, 2012.
Prior Publication US 2022/0403411 A1, Dec. 22, 2022
Int. Cl. A01H 5/10 (2018.01); A01H 1/00 (2006.01); A01H 6/46 (2018.01); C07K 14/415 (2006.01); C12N 15/82 (2006.01)
CPC C12N 15/8289 (2013.01) [A01H 5/10 (2013.01); A01H 6/4678 (2018.05); C07K 14/415 (2013.01); C12N 15/8287 (2013.01)] 7 Claims
 
1. A method of restoring male fertility to a mutated MS45 male-sterile wheat plant, wherein said method comprises introducing into said wheat plant a polynucleotide operably linked to a promoter that drives expression in a wheat plant, said polynucleotide comprising a nucleotide sequence selected from the group consisting of:
(a) a nucleotide sequence comprising SEQ ID NO: 13; and
(b) a nucleotide sequence comprising at least 95% sequence identity to SEQ ID NO: 13; and
expressing said polynucleotide in the wheat plant to confer male fertility to the mutated MS45 male-sterile wheat plant, wherein said polynucleotide is linked to a heterologous polynucleotide encoding a marker.