CPC C12Q 1/60 (2013.01) [C12Q 1/32 (2013.01); C12Q 1/44 (2013.01); G01N 2333/902 (2013.01); G01N 2333/918 (2013.01); G01N 2405/00 (2013.01)] | 18 Claims |
1. A method for measuring cholesterol in low-density lipoprotein in a sample, the method comprising:
mixing the sample and an aqueous solvent which comprises:
(i) a combination of cholesterol ester hydrolase and cholesterol oxidase or (ii) a combination of cholesterol ester hydrolase, an oxidized coenzyme and cholesterol dehydrogenase,
one or more surfactants selected from the group consisting of a polyoxyethylene alkyl ether and a polyoxyethylene polyoxypropylene alkyl ether,
a polyoxyethylene polyoxypropylene copolymer and
a polyoxypropylene derivative not comprising any polyoxyethylene in a molecule, to react cholesterol in low-density lipoprotein in the sample with (i) the combination of cholesterol ester hydrolase and cholesterol oxidase or (ii) the combination of cholesterol ester hydrolase, an oxidized coenzyme and cholesterol dehydrogenase; and
measuring a substance formed or consumed in the reaction from the low-density lipoprotein,
with the proviso that the aqueous solvent does not comprise any surfactant having an alkylphenol structure.
|