CPC C12N 5/0657 (2013.01) [G01N 33/5088 (2013.01); G09B 23/306 (2013.01); C12N 2500/02 (2013.01); C12N 2501/115 (2013.01); C12N 2529/00 (2013.01); C12N 2533/30 (2013.01)] | 12 Claims |
1. A method for culturing a mammalian heart organotypic slice, comprising:
culturing the slice in a culture medium, wherein the culture medium comprises fetal bovine serum (FBS), vascular endothelial cell growth factor (VEGF), fibroblast growth factor (FGF), and insulin-transferrin-selenium (ITS) and wherein the culture medium does not include 2, 3-butanedione monoxime (BDM) or any fatty acids added in addition to fatty acids present in the FBS; and
applying electrical stimulation to the slice while the heart slice is in culture, wherein the electrical stimulation has a frequency of 0.5 to 2 Hz and wherein the electrical stimulation has a voltage of 5 to 15 V, and wherein a viability of the heart slice measured on day 6 of culturing is not statistically significantly different from a viability of the heart slice measured on day 1 of culturing as measured by MTT assay.
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