US 12,168,794 B2
Methods for immunoassays using electrochemical measurement
Stanislav Sokolov, Oxford (GB); James Merotra, Oxford (GB); Konstantinos Psarros, Oxford (GB); Samuel Whitby, Oxford (GB); Adriano Dos Santos, Oxford (GB); and Flavio Cesar Bedatty Fernandes, Oxford (GB)
Assigned to Osler Diagnostics Limited, Oxford (GB)
Appl. No. 17/281,063
Filed by OSLER DIAGNOSTICS LIMITED, Oxford (GB)
PCT Filed Oct. 2, 2019, PCT No. PCT/GB2019/052769
§ 371(c)(1), (2) Date Mar. 29, 2021,
PCT Pub. No. WO2020/070486, PCT Pub. Date Apr. 9, 2020.
Claims priority of application No. 1816103 (GB), filed on Oct. 2, 2018.
Prior Publication US 2021/0332405 A1, Oct. 28, 2021
Int. Cl. G01N 27/327 (2006.01); C12Q 1/00 (2006.01); C12Q 1/28 (2006.01)
CPC C12Q 1/005 (2013.01) [C12Q 1/28 (2013.01); G01N 2333/4737 (2013.01)] 18 Claims
OG exemplary drawing
 
1. A method for detecting a binding event between an immobilised primary antibody and a target analyte-secondary antibody-enzyme complex using an electrochemical measurement technique, the method comprising:
incubating a target analyte, a secondary antibody and an enzyme label in a single solution;
contacting a primary antibody immobilised on an electrode surface with the single solution of the target analyte, the secondary antibody and the enzyme label to create a sandwich complex bound to the electrode surface;
contacting the sandwich complex with a substrate for the enzyme, wherein the substrate is converted by the enzyme into an insoluble precipitate on the electrode surface; and
measuring an electrochemical signal during and/or after the formation of the insoluble precipitate on the electrode surface.