US 12,168,784 B2
Method of co-expressing a sulfatase and a sulfatase modifying factor in a plant or plant cell
David N. Radin, Jonesboro, AR (US)
Assigned to BioStrategies LC, State University, AR (US)
Filed by BIOSTRATEGIES LC, State University, AR (US)
Filed on Feb. 15, 2022, as Appl. No. 17/651,106.
Application 17/651,106 is a continuation of application No. 15/325,661, abandoned, previously published as PCT/US2015/040030, filed on Jul. 10, 2015.
Claims priority of provisional application 62/023,571, filed on Jul. 11, 2014.
Prior Publication US 2022/0251522 A1, Aug. 11, 2022
Int. Cl. C12N 15/82 (2006.01); A61K 38/16 (2006.01); A61K 38/17 (2006.01); A61K 38/44 (2006.01); C07K 14/42 (2006.01); C12N 9/00 (2006.01); C12N 9/02 (2006.01); C12N 9/16 (2006.01)
CPC C12N 9/0051 (2013.01) [A61K 38/168 (2013.01); A61K 38/17 (2013.01); A61K 38/44 (2013.01); C07K 14/42 (2013.01); C12N 9/16 (2013.01); C12N 15/8257 (2013.01); C12Y 108/99 (2013.01); C12Y 301/06 (2013.01); C07K 2319/00 (2013.01); C07K 2319/02 (2013.01); C07K 2319/04 (2013.01)] 7 Claims
 
1. A method for producing an enzymatically active sulfatase fusion protein having sulfatase activity, and/or an enzymatically active mammalian sulfatase protein having sulfatase activity, comprising co-expressing in a plant or plant cell: (1) a polynucleotide encoding a mammalian sulfatase fusion protein, and/or a polynucleotide encoding a mammalian sulfatase protein; and (2) a polynucleotide encoding a mammalian sulfatase modifying factor 1 (SUMF1) protein or a SUMF1 fusion protein, wherein the expressed sulfatase fusion protein and/or the expressed mammalian sulfatase protein produced by the method is enzymatically activated in the plant or plant cell by the expressed SUMF1 protein or the expressed SUMF1 fusion protein,
wherein the expressed mammalian sulfatase fusion protein and/or the expressed mammalian sulfatase protein comprises a signal peptide operably linked thereto, and wherein the signal peptide comprises the amino acid sequence of SEQ ID NO: 37.