US 12,168,798 B2
Sample preparation, processing and analysis systems
David Eberhart, Santa Clara, CA (US); Yuan Li, Dublin, CA (US); James Ogg, Sunnyvale, CA (US); Ezra Van Gelder, Palo Alto, CA (US); Stephen J. Williams, San Carlos, CA (US); Timothy Woudenberg, San Francisco, CA (US); Dean S. Burgi, Sunnyvale, CA (US); and William D. Nielsen, San Jose, CA (US)
Assigned to INTEGENX. INC., Pleasanton, CA (US)
Filed by Integenx Inc., Pleasanton, CA (US)
Filed on Dec. 11, 2020, as Appl. No. 17/119,874.
Application 17/119,874 is a division of application No. 14/381,179, granted, now 10,865,440, previously published as PCT/US2013/028462, filed on Feb. 28, 2013.
Application 14/381,179 is a continuation in part of application No. 13/656,503, filed on Oct. 19, 2012, granted, now 8,894,946, issued on Nov. 25, 2014.
Claims priority of provisional application 61/703,194, filed on Sep. 19, 2012.
Claims priority of provisional application 61/696,809, filed on Sep. 5, 2012.
Claims priority of provisional application 61/691,242, filed on Aug. 20, 2012.
Claims priority of provisional application 61/674,295, filed on Jul. 20, 2012.
Claims priority of provisional application 61/671,592, filed on Jul. 13, 2012.
Claims priority of provisional application 61/664,726, filed on Jun. 26, 2012.
Claims priority of provisional application 61/654,749, filed on Jun. 1, 2012.
Claims priority of provisional application 61/641,120, filed on May 1, 2012.
Claims priority of provisional application 61/610,977, filed on Mar. 14, 2012.
Claims priority of provisional application 61/605,169, filed on Feb. 29, 2012.
Claims priority of provisional application 61/550,364, filed on Oct. 21, 2011.
Prior Publication US 2021/0277445 A1, Sep. 9, 2021
Int. Cl. C12Q 1/686 (2018.01); B01L 3/00 (2006.01); B01L 7/00 (2006.01); G01N 27/447 (2006.01); G01N 35/00 (2006.01)
CPC C12Q 1/686 (2013.01) [B01L 3/502715 (2013.01); B01L 3/527 (2013.01); B01L 7/52 (2013.01); B01L 3/5029 (2013.01); B01L 2200/025 (2013.01); B01L 2200/028 (2013.01); B01L 2200/04 (2013.01); B01L 2200/0647 (2013.01); B01L 2200/0684 (2013.01); B01L 2200/143 (2013.01); B01L 2300/023 (2013.01); B01L 2300/027 (2013.01); B01L 2300/0672 (2013.01); B01L 2300/123 (2013.01); B01L 2400/0421 (2013.01); B01L 2400/043 (2013.01); B01L 2400/0478 (2013.01); B01L 2400/0616 (2013.01); G01N 27/44704 (2013.01); G01N 2035/00158 (2013.01)] 21 Claims
OG exemplary drawing
 
1. A microfluidic device, comprising:
a reaction chamber;
a microfluidic channel in fluidic communication with the reaction chamber; and
a vial comprising:
a compartment extending between a first end and a second end opposite the first end, wherein the first end is above the second end;
an exit port at the second end of the compartment and in fluidic communication with the microfluidic channel;
a first movable stopper sealing the compartment from fluidic communication with an external environment through the first end; and
a second movable stopper sealing the compartment from fluidic communication with the microfluidic channel through the exit port;
wherein, in a state of the first movable stopper sealing the compartment and the second movable stopper sealing the compartment, the compartment is configured to hold a fluid composition between the first movable stopper and the second movable stopper; and
wherein the first movable stopper and the second movable stopper are configured to move relative to each other such that, in response to force applied to the first movable stopper to move the first movable stopper toward the second moveable stopper, the second moveable stopper is actuatable to unseal and place the compartment in fluidic communication with the microfluidic channel through the exit port.