US 12,168,773 B2
Engineered phosphoenolpyruvate carboxylase enzymes
Paul B. Larsen, Riverside, CA (US)
Assigned to The Regents of the University of California, Oakland, CA (US)
Appl. No. 17/601,913
Filed by The Regents of the University of California, Oakland, CA (US)
PCT Filed Apr. 10, 2020, PCT No. PCT/US2020/027746
§ 371(c)(1), (2) Date Oct. 6, 2021,
PCT Pub. No. WO2020/210687, PCT Pub. Date Oct. 15, 2020.
Claims priority of provisional application 62/832,727, filed on Apr. 11, 2019.
Prior Publication US 2022/0145319 A1, May 12, 2022
Int. Cl. C12N 15/82 (2006.01); C12N 9/88 (2006.01)
CPC C12N 15/8271 (2013.01) [C12N 9/88 (2013.01); C12N 15/8243 (2013.01); C12N 15/8262 (2013.01); C12Y 401/01031 (2013.01); C12N 2820/002 (2013.01); C12N 2820/007 (2013.01)] 6 Claims
 
1. A method for producing a maize plant expressing a variant phosphoenolpyruvate carboxylase (PEPC), comprising:
(a) introducing a clustered regularly interspaced short palindromic repeats (CRISPR) associated protein (Cas9) genome-editing system into a maize plant cell to form a transformed maize plant cell comprising a nucleic acid encoding the variant PEPC comprising (i) an amino acid substitution selected from the group consisting of A651V, G678S, and T778I in the consensus sequence of SEQ ID NO:8, and/or (ii) an amino acid substitution selected from the group consisting of A776S and R886G in the consensus sequence of SEQ ID NO:8; and
(b) regenerating a maize plant from the transformed plant cell,
wherein the maize plant expresses the variant PEPC and has enhanced aluminum resistance as compared to a control maize plant that does not express the variant PEPC.