US 12,169,165 B2
Antigen protection method for enabling delayed sample processing
Bipin Gupta, Pleasanton, CA (US); and Marc Key, Ojai, CA (US)
Assigned to Diagnostic BioSystems, Pleasanton, CA (US); and Marc Key, Ojai, CA (US)
Filed by Diagnostic BioSystems, Pleasanto, CA (US); and Marc Key, Ojai, CA (US)
Filed on Feb. 11, 2020, as Appl. No. 16/787,595.
Claims priority of provisional application 62/909,345, filed on Oct. 2, 2019.
Prior Publication US 2021/0102871 A1, Apr. 8, 2021
Int. Cl. G01N 1/28 (2006.01); G01N 1/30 (2006.01); G01N 1/36 (2006.01); G01N 1/44 (2006.01)
CPC G01N 1/28 (2013.01) [G01N 1/2806 (2013.01); G01N 1/30 (2013.01); G01N 1/36 (2013.01); G01N 1/44 (2013.01)] 7 Claims
 
1. An antigen protection method for enabling delayed sample processing, the method comprising the steps performed in the following sequential order of:
fixing a sample;
embedding the sample, wherein the sample is not fixed again after the step of embedding;
sectioning the sample and placing a section of the sample in contact with a microscope slide to produce a sectioned sample;
de-embedding the sectioned sample;
performing target retrieval on the sectioned sample;
applying a protecting reagent onto the sectioned sample;
drying the protecting reagent; and
performing target visualization on the sectioned sample, wherein a period of time is allowed to elapse between the completion of the step of drying the protecting reagent and the beginning of the step of performing target visualization on the sectioned sample.